A membrane associated mCherry fluorescent reporter line for studying vascular remodeling and cardiac function during murine embryonic development.
A membrane associated mCherry fluorescent reporter line for studying vascular remodeling and cardiac function during murine embryonic development.
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DOI：
10.1002/ar.20821
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发表时间：
2009-03
影响因子：
2
通讯作者：
Dickinson, Mary E.
中科院分区：
文献类型：
--
作者：
Larina, Irina V.;Shen, Wei;Kelly, Olivia G.;Hadjantonakis, Anna-Katerina;Baron, Margaret H.;Dickinson, Mary E.
关键词：
心血管系统的发育是一个高度动态的过程，依赖于调节增殖、分化、迁移、细胞-细胞和细胞-基质相互作用的多种信号传导途径。为了表征小鼠心血管系统形成过程中的细胞和组织动力学，我们产生了一种新的转基因小鼠品系Tg（Flk 1 Myr-mCherry），其中内皮细胞膜被红色荧光蛋白mCherry明亮标记。Tg（Flk 1 Myr-mCherry）小鼠存活，可生育，未表现出任何发育异常。高水平的mCherry在胚胎内皮和内皮细胞中表达，并且在成年动物的毛细血管中也观察到表达。将荧光蛋白靶向细胞膜允许亚细胞成像和细胞跟踪。通过获得显微镜下培养的活胚胎的共聚焦时间间隔，我们证明了新产生的转基因模型漂亮地突出了血管丛形成过程中内皮细胞的发芽行为。我们还使用来自该品系的胚胎对跳动的胚胎小鼠心脏中的内皮细胞进行成像，表明Tg（Flk 1 Myr-mCherry）小鼠适合于表征心脏动力学。此外，当与先前描述的Tg（Flk 1 β H2 B-EYFP）系组合时，除了细胞结构之外，还揭示了细胞数量，使得可以确定单个内皮细胞如何对血管的结构做出贡献。
The development of the cardiovascular system is a highly dynamic process dependent on multiple signaling pathways regulating proliferation, differentiation, migration, cell-cell and cell-matrix interactions. To characterize cell and tissue dynamics during the formation of the cardiovascular system in mice, we generated a novel transgenic mouse line, Tg(Flk1∷myr-mCherry), in which endothelial cell membranes are brightly labeled with mCherry, a red fluorescent protein. Tg(Flk1∷myr-mCherry) mice are viable, fertile and do not exhibit any developmental abnormalities. High levels of mCherry are expressed in the embryonic endothelium and endocardium, and expression is also observed in capillaries in adult animals. Targeting of the fluorescent protein to the cell membrane allows for sub-cellular imaging and cell tracking. By acquiring confocal time lapses of live embryos cultured on the microscope stage, we demonstrate that the newly generated transgenic model beautifully highlights the sprouting behaviors of endothelial cells during vascular plexus formation. We have also used embryos from this line to imaging the endocardium in the beating embryonic mouse heart, showing that Tg(Flk1∷myr-mCherry) mice are suitable for the characterization of cardio dynamics. Furthermore, when combined with the previously described Tg(Flk1∷H2B-EYFP) line, cell number in addition to cell architecture is revealed, making it possible to determine how individual endothelial cells contribute to the structure of the vessel.
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影响因子：
2.5
作者：
Liebling, Michael;Forouhar, Arian S.;Dickinson, Mary E.
通讯作者：
Dickinson, Mary E.
DOI：
10.1002/bdrc.20021
发表时间：
2004-09-01
期刊：
Birth defects research. Part C, Embryo today : reviews
影响因子：
--
作者：
Kulesa, Paul M
通讯作者：
Kulesa, Paul M
影响因子：
64.8
作者：
Risau, W
通讯作者：
Risau, W
影响因子：
1.5
作者：
Fraser, ST;Hadjantonakis, AK;Baron, MH
通讯作者：
Baron, MH
影响因子：
4.6
作者：
Lucitti, Jennifer L.;Jones, Elizabeth A. V.;Dickinson, Mary E.
通讯作者：
Dickinson, Mary E.