Genome Survey Sequencing of the Mole Cricket Gryllotalpa orientalis.
Genome Survey Sequencing of the Mole Cricket Gryllotalpa orientalis.
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东方蝼蛄的基因组调查测序
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蟋蟀（Gryllotalpa orientalis）是一种进化、药用和农业上重要的昆虫，栖息在地下环境中，分布在全球。本研究通过流式细胞术和基于低覆盖测序的k-mer测量了基因组大小，并鉴定了核重复元件。基于流式细胞术、3.17 Gb和3.77 Gb的两种k-mer方法估计的单倍体基因组大小分别为3.14 Gb、3.17 Gb和3.77 Gb，这与先前报道的其他物种Ensifera亚目的范围相当。然而，大量的重复序列无法被标注为特定的重复元件族。在被注释的重复元件中，I-LINE类反转录转座子元件是最常见的家族，比卫星和I-LTR类更丰富。这些研究结果可用于东方木的分类研究和全基因组测序，以提高对东方木生物学的认识。
The mole cricket Gryllotalpa orientalis is an evolutionarily, medicinal, and agriculturally significant insect that inhabits underground environments and is distributed globally. This study measured genome size by flow cytometry and k-mer based on low-coverage sequencing, and nuclear repetitive elements were also identified. The haploid genome size estimate is 3.14 Gb by flow cytometry, 3.17 Gb, and 3.77 Gb-based two k-mer methods, respectively, which is well within the range previously reported for other species of the suborder Ensifera. 56% of repetitive elements were found in G. orientalis, similar to 56.83% in Locusta migratoria. However, the great size of repetitive sequences could not be annotated to specific repeat element families. For the repetitive elements that were annotated, Class I-LINE retrotransposon elements were the most common families and more abundant than satellite and Class I-LTR. These results based on the newly developed genome survey could be used in the taxonomic study and whole genome sequencing to improve the understanding of the biology of G. orientalis.
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