Olfactory bulb acetylcholine release dishabituates odor responses and reinstates odor investigation.
Olfactory bulb acetylcholine release dishabituates odor responses and reinstates odor investigation.
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DOI：
10.1038/s41467-018-04371-w
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发表时间：
2018-05-14
影响因子：
16.6
通讯作者：
Fletcher ML
中科院分区：
文献类型：
--
作者：
Ogg MC;Ross JM;Bendahmane M;Fletcher ML
习惯化和非习惯化调节神经资源和行为的意义分配到传入刺激的感觉系统。我们在小鼠嗅球（OB）中表征了这些过程，并揭示了OB乙酰胆碱（ACh）在生理和行为嗅觉失调中的作用。我们在清醒和麻醉小鼠中使用钙显像来确定OB肾小球在长时间气味表现期间习惯化的时间过程和程度。此外，我们开发了一种新的行为调查范式，以确定长时间的气味输入如何影响气味显著性。我们发现，在长时间的气味呈现过程中，通过电或光遗传刺激来控制OB - ACh的释放，可以快速调节习惯性的肾小球气味反应和气味显著性，导致小鼠突然调查以前被忽视的气味。为了证明这一效应的行为学有效性，我们表明，改变视觉环境会导致气味调查行为的不习惯化，这一行为被OB中的胆碱能拮抗剂阻断。习惯化会降低神经对长时间不相关刺激的反应，而当遇到显著刺激时，不习惯化会逆转这些影响。在这里，作者证明了乙酰胆碱参与了小鼠嗅球的气味异常反应。
Habituation and dishabituation modulate the neural resources and behavioral significance allocated to incoming stimuli across the sensory systems. We characterize these processes in the mouse olfactory bulb (OB) and uncover a role for OB acetylcholine (ACh) in physiological and behavioral olfactory dishabituation. We use calcium imaging in both awake and anesthetized mice to determine the time course and magnitude of OB glomerular habituation during a prolonged odor presentation. In addition, we develop a novel behavioral investigation paradigm to determine how prolonged odor input affects odor salience. We find that manipulating OB ACh release during prolonged odor presentations using electrical or optogenetic stimulation rapidly modulates habituated glomerular odor responses and odor salience, causing mice to suddenly investigate a previously ignored odor. To demonstrate the ethological validity of this effect, we show that changing the visual context can lead to dishabituation of odor investigation behavior, which is blocked by cholinergic antagonists in the OB. Habituation reduces neural responsiveness to prolonged irrelevant stimuli and dishabituation reverses these effects when a salient stimulus is encountered. Here the authors demonstrate that acetylcholine is involved in dishabituating odor responses in the mouse olfactory bulb.
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影响因子：
2.5
作者：
Fletcher, Max L.;Masurkar, Arjun V.;Chen, Wei R.
通讯作者：
Chen, Wei R.
影响因子：
25
作者：
Cazakoff, Brittany N.;Lau, Billy Y. B.;Shea, Stephen D.
通讯作者：
Shea, Stephen D.
影响因子：
1.9
作者：
Chaudhury, Dipesh;Manella, Laura;Arellanos, Adolfo;Escanilla, Olga;Cleland, Thomas A.;Linster, Christiane
通讯作者：
Linster, Christiane
影响因子：
3.4
作者：
FORT, P;KHATEB, A;JONES, BE
通讯作者：
JONES, BE
延髓乙酰胆碱增强神经和感知气味辨别能力。
DOI：
10.1523/jneurosci.4036-08.2009
发表时间：
2009-01-07
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Chaudhury D;Escanilla O;Linster C
通讯作者：
Linster C