Molecular identification of two Eimeria species, E. uekii and E. raichoi as type B, in wild Japanese rock ptarmigans, Lagopus muta japonica.
Molecular identification of two Eimeria species, E. uekii and E. raichoi as type B, in wild Japanese rock ptarmigans, Lagopus muta japonica.
复制标题
DOI：
10.1016/j.ijppaw.2018.06.005
复制
发表时间：
2018-12
期刊：
影响因子：
--
通讯作者：
Ushida K
中科院分区：
文献类型：
--
作者：
Matsubayashi M;Tsuchida S;Kobayashi A;Shibahara T;Nakamura H;Murata K;Ushida K
迄今为止，已在野生日本岩雷鸟 (Lagopus muta japonica) 中从形态上鉴定出两种类型的艾美耳球虫寄生虫（E. uekii 和 B 型）。尽管据报道这些寄生虫的患病率很高，但尚未进行遗传分析。我们首先通过 18S rRNA 和线粒体细胞色素 c 氧化酶亚基 I 基因区域的遗传分析阐明了两个艾美耳球虫分离株的系统发育位置。因此，在检查的 61 个样本中，分别有 21 个和 11 个样本呈 E. uekii 和 B 型阳性。此外，夏季感染率有所上升。分子分析显示，两种艾美耳球虫分离株都形成了自己的簇； E. uekii 属于鸡艾美耳球虫分支，B 型属于火鸡艾美耳球虫分支。根据我们在本研究中的发现和之前的数据，我们在此建议 B 型为 E. raichoi。这些遗传数据将有助于进行详细分类并了解这些寄生虫对保护濒临灭绝的日本岩雷鸟的影响。分子分析显示，从日本岩雷鸟中分离出来的艾美耳球虫形成了自己的进化枝。该物种在系统发育上与有毒的鸡艾美耳球虫属密切相关。根据形态学数据和序列，B 型艾美耳球虫被提议为新种 E. raichoi。这些寄生虫可能是保护濒临灭绝的鸟类的关键因素。
Thus far, two types of Eimeria parasites (E. uekii and type B) have been morphologically identified in wild Japanese rock ptarmigans, Lagopus muta japonica. Although high prevalences were reported for these parasites, genetic analyses have not been conducted. We first clarified the phylogenetic positions of two eimerian isolates using genetic analyses of 18S rRNA and mitochondrial cytochrome c oxidase subunit I gene regions. Consequently, of 61 samples examined, 21 and 11 samples were positive for E. uekii and type B, respectively. Additionally, the infection rate increased in the summer. Molecular analyses revealed both Eimeria isolates formed their own clusters; E. uekii was included in clades of chicken Eimeria and type B was include in clades of turkey Eimeria. Based on our findings in this study and previous data, we herein propose type B as E. raichoi. These genetic data will be helpful to conduct detailed classification and understand the impact of these parasites for conservation of endangered Japanese rock ptarmigans. Molecular analyses revealed Eimeria isolates from Japanese rock ptarmigans formed own clades. The species was phylogenetically closely related to virulent chicken Eimeria spp. Based on morphological data and sequences, Eimeria type B was proposed as a new species E. raichoi. These parasites could be the key factor for conservation of the endangered birds.
登录
查看更多内容
影响因子：
2.4
作者：
LONG, PL;MILLARD, BJ;SHIRLEY, MW
通讯作者：
SHIRLEY, MW
影响因子：
2
作者：
Skirnisson, K.;Thorarinsdottir, S. Th.
通讯作者：
Thorarinsdottir, S. Th.
影响因子：
2.1
作者：
Yang, Rongchang;Brice, Belinda;Ryan, Una
通讯作者：
Ryan, Una
影响因子：
2
作者：
Jinneman, KC;Wetherington, JH;Wekell, MM
通讯作者：
Wekell, MM
影响因子：
1.2
作者：
Murata, Koichi;Tamada, Asumi;Asakawa, Mitsuhiko
通讯作者：
Asakawa, Mitsuhiko