Effects of Aroclor 1254 on in vivo oocyte maturation in the mouse.
Effects of Aroclor 1254 on in vivo oocyte maturation in the mouse.
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Aroclor 1254 对小鼠体内卵母细胞成熟的影响
DOI：
10.1371/journal.pone.0102064
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Miao Y
中科院分区：
文献类型：
--
作者：
Liu S;Jiang L;Meng X;Han X;Cheng D;Zhang T;Miao Y
多氯联苯是一种稳定的亲脂化合物，在环境和食物链中积累。尽管一些研究提供了多氯联苯对生殖功能有不利影响的证据，但这些结果大多来自体外模型。因此，我们研究了Aroclor 1254(一种商品多氯联苯混合物)处理对小鼠卵母细胞体内成熟和发育潜力的影响。在本研究中，雌性ICR小鼠分别给予不同剂量(12.5、25和50 mg/kg)的Aroclor 1254(商品多氯联苯混合物)，每72小时一次，连续9天。在Aroclor 1254进行了三次处理后，小鼠在最后一次暴露后一天进行超数排卵，收集卵母细胞。研究了Aroclor 1254对卵母细胞成熟、受精和植入前胚胎发育的影响。免疫荧光染色的卵母细胞在共聚焦显微镜下观察，以评估Aroclor 1254对纺锤体形态的影响。观察卵丘-卵母细胞复合体中孤雌生殖激活和卵丘细胞凋亡的发生率。在体内暴露于不同剂量的Aroclor 1254的卵母细胞，其体外生长潜能显著降低，纺锤体结构异常，孤雌激活受到抑制。此外，暴露于Aroclor 1254后，卵丘细胞的凋亡率增加。这些结果可为环境污染物引起的不孕不育或流产等生殖疾病的治疗提供参考。
Polychlorinated biphenyls (PCBs) are stable, lipophilic compounds that accumulate in the environment and in the food chain. Though some studies provided evidence that PCBs had adverse effects on reproductive function, most of these results were from in vitro models. Therefore we investigated the effect of Aroclor 1254 (a commercial PCBs mixture) treatments on in vivo maturation and developmental potential of mouse oocytes. In the present study, female ICR mice were treated with different doses (12.5, 25 and 50 mg/kg) of Aroclor 1254 (a commercial PCB mixture) once every 72 hours by intraperitoneal injection for 9 days. After three treatments of Aroclor 1254, the mice were superovulated to collect oocytes one day after the last exposure. The effects of Aroclor 1254 on oocyte maturation, fertilization, and preimplantation embryonic development were investigated. Immunofluorescence-stained oocytes were observed under a confocal microscope to assess the effects of Aroclor 1254 on spindle morphology. Parthenogenic activation and the incidence of cumulus apoptosis in cumulus-oocyte complexes were observed as well. Oocytes exposed to different doses of Aroclor 1254 in vivo were associated with a significant decrease in outgrowth potential, abnormal spindle configurations, and the inhibition of parthenogenetic activation of ovulated oocytes. Furthermore, the incidence of apoptosis in cumulus cells was increased after exposed to Aroclor 1254. These results may provide reference for the treatment of reproductive diseases such as infertility or miscarriage caused by environmental contaminants.
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影响因子：
2.8
作者：
Katz-Jaffe, M. G.;McCallie, B. R.;Gardner, D. K.
通讯作者：
Gardner, D. K.
影响因子：
2.8
作者：
Méo, SC;Yamazaki, W;Garcia, JM
通讯作者：
Garcia, JM
影响因子：
4
作者：
Guillette, LJ;Brock, JW;Woodward, AR
通讯作者：
Woodward, AR
影响因子：
4.5
作者：
Cai, Jiali;Wang, Chonggang;Zuo, Zhenghong
通讯作者：
Zuo, Zhenghong
影响因子：
2.5
作者：
Campagna, C;Bailey, JL;Maddox-Hyttel, P
通讯作者：
Maddox-Hyttel, P