EXPLORING GENE INDICATORS TO PREDICT SUSCEPTIBILITY TO SCLEROTINIA SCLEROTIORUM IN BRASSICA PLANTS
EXPLORING GENE INDICATORS TO PREDICT SUSCEPTIBILITY TO SCLEROTINIA SCLEROTIORUM IN BRASSICA PLANTS
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探索基因指标来预测芸苔属植物对核盘菌的易感性
DOI:
10.4454/jpp.v99i3.3939
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发表时间:
2017-10
影响因子:
2.2
通讯作者:
Wei Qian
中科院分区:
文献类型:
--
作者:
Yuehua Li;Yijuan Ding;Chaoguo Shao;Jiaqin Mei;Wei Qian
Sclerotinia stem rot caused by Sclerotinia sclerotiorum is a devastating disease in Brassica spp. Here we proposed a method to indicate the susceptibility of Brassica spp. or Brassica genus against S. sclerotiorum by detecting expression of a gene from host with qRT-PCR technique. After filtering a set of transcriptome data from B. oleracea leaf infected by S. sclerotiorum, twelve genes that exhibited successive increase or decrease expression after inoculation were chosen to screen the dynamic expression changes in S. sclerotiorum-inoculated B. napus leaf. Among the genes tested, Bol024541 showed a continuous increase from the outer region to the inoculation site from 12 to 36 hours after infection (hai). No significant difference was found in qRT-PCR for the expression of Bol024541 in the samples added with various amount of cDNA of S. sclerotiorum, and significant linear correlation (r = 0.921, P < 0.01) was detected for the expression of Bol024541 at 12 hai with the lesion size at 72 hai among six Brassica accessions. Our data suggest that Bol024541 may be considered as an indicator to predict susceptibility to S. sclerotiorum in Brassica spp. via qRT-PCR.
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DOI:
10.1007/s00122-002-1171-2
发表时间:
2003-02
期刊:
Theor. Appl. GenetSCI源刊物,
影响因子:
--
作者:
赵建伟
通讯作者:
赵建伟
影响因子:
4.4
作者:
Dang ZH;Zheng LL;Wang J;Gao Z;Wu SB;Qi Z;Wang YC
通讯作者:
Wang YC
影响因子:
5.4
作者:
Jianwei Zhao;J. Udall;P. Quijada;C. Grau;J. Meng;T. Osborn
通讯作者:
Jianwei Zhao;J. Udall;P. Quijada;C. Grau;J. Meng;T. Osborn
影响因子:
7.2
作者:
Clarke, A;Desikan, R;Neill, SJ
通讯作者:
Neill, SJ
影响因子:
3.2
作者:
Koch, S.;Dunker, S.;von Tiedemann, A.
通讯作者:
von Tiedemann, A.