EXPLORING GENE INDICATORS TO PREDICT SUSCEPTIBILITY TO SCLEROTINIA SCLEROTIORUM IN BRASSICA PLANTS

EXPLORING GENE INDICATORS TO PREDICT SUSCEPTIBILITY TO SCLEROTINIA SCLEROTIORUM IN BRASSICA PLANTS
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探索基因指标来预测芸苔属植物对核盘菌的易感性

DOI:
10.4454/jpp.v99i3.3939
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发表时间:
2017-10
影响因子:
2.2
通讯作者:
Wei Qian
Wei Qian
中科院分区:
农林科学4区
文献类型:
--
作者:
Yuehua Li;Yijuan Ding;Chaoguo Shao;Jiaqin Mei;Wei Qian

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由核盘菌引起的菌核病是甘蓝型油菜的一种毁灭性病害。在此,我们提出了一种指示甘蓝属植物感病程度的方法。或利用qRT-PCR技术检测寄主基因的表达。筛选了一组甘蓝型油菜感染核盘菌后的转录组数据,筛选出12个在接种核盘菌后表达连续增加或减少的基因,用于筛选接种核盘菌后甘蓝型油菜叶片中的动态表达变化。在所检测的基因中,Bol024541在感染后12~36小时(HAI)表现出从外区到接种部位的持续增加。不同核盘菌量的供试材料中Bol024541的表达差异不显著,6个甘蓝型油菜材料中Bol024541在12HAI时的表达与72HAI时的病斑大小呈显著的线性相关(r=0.921,P&lt0.01)。我们的数据表明,Bol024541可以被认为是预测油菜对核盘菌敏感性的一个指标。通过qRT-PCR。
Sclerotinia stem rot caused by Sclerotinia sclerotiorum is a devastating disease in Brassica spp. Here we proposed a method to indicate the susceptibility of Brassica spp. or Brassica genus against S. sclerotiorum by detecting expression of a gene from host with qRT-PCR technique. After filtering a set of transcriptome data from B. oleracea leaf infected by S. sclerotiorum, twelve genes that exhibited successive increase or decrease expression after inoculation were chosen to screen the dynamic expression changes in S. sclerotiorum-inoculated B. napus leaf. Among the genes tested, Bol024541 showed a continuous increase from the outer region to the inoculation site from 12 to 36 hours after infection (hai). No significant difference was found in qRT-PCR for the expression of Bol024541 in the samples added with various amount of cDNA of S. sclerotiorum, and significant linear correlation (r = 0.921, P < 0.01) was detected for the expression of Bol024541 at 12 hai with the lesion size at 72 hai among six Brassica accessions. Our data suggest that Bol024541 may be considered as an indicator to predict susceptibility to S. sclerotiorum in Brassica spp. via qRT-PCR.
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