Dynamic metabolite alterations of Portunus trituberculatus during larval development
Dynamic metabolite alterations of Portunus trituberculatus during larval development
复制标题
三疣梭子蟹幼虫发育过程中的动态代谢变化
DOI：
10.1007/s00343-019-7268-0
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发表时间：
2018-11
影响因子：
1.6
通讯作者：
Mu Changkao
中科院分区：
文献类型：
--
作者：
Shi Ce;Zeng Tinglan;Li Ronghua;Wang Chunlin;Ye Yangfang;Mu Changkao
在三疣梭子蟹幼体发育过程中，从蜕皮到大眼幼体阶段经常发生大量死亡。幼体若在蚤状幼体阶段的营养累积不足，可能无法发育成幼蟹。然而，对三疣梭子蟹幼体的营养信息，如初级代谢产物的研究却很少。本研究旨在了解三疣梭子蟹早期发育阶段的代谢特征。利用核磁共振氢谱结合多变量数据分析方法研究了三疣梭子蟹幼虫发育过程中代谢产物的变化。结果表明，总代谢物的趋势表现为从1号到3号的上升，然后从4号到大眼幼体的下降和恢复在第一个幼体阶段。在蚤状幼体4和大眼幼体阶段总代谢物的大规模消耗表明代谢活性的深度抑制，这可能与从蜕皮到大眼幼体阶段的大量死亡有关。这些结果为了解三疣梭子蟹幼体发育过程提供了重要的代谢信息，也为了解三疣梭子蟹早期发育阶段的营养需求提供了重要线索。
A mass mortality often occurs from molting to the megalopa stage during the larval development of the swimming crab Portunus trituberculatus. Larvae with insufficient nutrient accumulation during the zoeal stages are probably unable to develop into juvenile swimming crabs. However, the nutritional information such as the primary metabolites is scarce for P. trituberculatus larvae. The aim of this work is to obtain an insight into the metabolite traits of P. trituberculatus at early developmental stages. 1 H nuclear magnetic resonance spectroscopy coupled with multivariate data analysis was used to determine how the metabolite profiles shift during larval development in P. trituberculatus. Our results show that the trend of total metabolites exhibited a rise from zoea 1 to zoea 3, followed by a drop from zoea 4 to megalopa and recovery during the first juvenile stage. A large-scale depletion of total metabolites in the zoea 4 and megalopa stages suggests a deep depression of metabolic activity, which may be linked to the mass mortality from molting to the megalopa stage. These findings provided essential metabolic information about the larval development of P. trituberculatus and important clues for understanding the nutritional requirements of P. trituberculatus in early developmental stages.
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影响因子：
4.5
作者：
M. Suprayudi;T. Takeuchi;K. Hamasaki
通讯作者：
M. Suprayudi;T. Takeuchi;K. Hamasaki
DOI：
10.1016/s1095-6433(02)00182-4
发表时间：
2002-11
期刊：
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
影响因子：
--
作者：
P. Yancey;W. R. Blake;J. Conley
通讯作者：
P. Yancey;W. R. Blake;J. Conley
影响因子：
2.4
作者：
M. Andrés;A. Estévez;F. Hontoria;G. Rotllant
通讯作者：
M. Andrés;A. Estévez;F. Hontoria;G. Rotllant
影响因子：
4.5
作者：
May-Helen Holme;C. Zeng;P. Southgate
通讯作者：
May-Helen Holme;C. Zeng;P. Southgate
DOI：
--
发表时间：
2006
期刊：
--
影响因子：
--
作者：
R. A. V. D. Berg;H. Hoefsloot;J. Westerhuis;A. Smilde;M. Werf
通讯作者：
R. A. V. D. Berg;H. Hoefsloot;J. Westerhuis;A. Smilde;M. Werf