Vaginal Inoculation of Uropathogenic Escherichia coli during Estrus Leads to Genital and Renal Colonization.
Vaginal Inoculation of Uropathogenic Escherichia coli during Estrus Leads to Genital and Renal Colonization.
复制标题
DOI：
10.1128/iai.00532-21
复制
发表时间：
2022-04-21
影响因子：
3.1
通讯作者：
Subashchandrabose, Sargurunathan
中科院分区：
文献类型：
--
作者：
Robinson, Christen K.;Saenkham-Huntsinger, Panatda;Hanson, Braden S.;Adams, L. Garry;Subashchandrabose, Sargurunathan
尿路感染（UTI）是最常见的细菌感染之一，尤其是在妇女、儿童和老年人中。尿路致病性大肠杆菌（UPEC）是尿路感染的主要病原体。在广泛使用的UTI小鼠模型中，将尿路病原体直接滴注到膀胱中，绕过下泌尿生殖道。我们评估了阴道接种UPEC是否会导致UTI以及发情周期的阶段如何影响小鼠中的细菌定植。通过阴道细胞学鉴定发情前期、发情期、发情后期和间情期的小鼠，并在阴道中接种UPEC。感染后1天对小鼠实施安乐死，并对泌尿生殖道、肝脏和脾脏中的细菌负荷进行计数。发情期小鼠在所有器官中表现出最高和最一致的UPEC负荷，膀胱除外。阴道接种导致膀胱定植在UPEC株特异性的方式。相反，经尿道接种UPEC导致膀胱定植。重要的是，通过两种途径接种导致阴道和子宫定植，并伴随全身性传播到脾脏和肝脏。阴道接种后2周内细菌定植的动力学在泌尿生殖道中相当。组织切片显示阴道滴注UPEC后诱导阴道炎和膀胱炎。总之，发情期小鼠阴道接种UPEC代表了一种研究肾脏和生殖道感染以及泌尿生殖道全身传播的新方法。我们的研究结果表明，雌激素可以启动泌尿生殖道，为UPEC定植创造有利的环境。
Urinary tract infection (UTI) is one of the most prevalent bacterial infections, particularly in women, children, and the elderly. Uropathogenic Escherichia coli (UPEC) is the predominant etiological agent of UTI. Uropathogens are directly instilled in the urinary bladder, bypassing the lower urogenital tract, in the widely used murine model of UTI. We assessed whether vaginal inoculation of UPEC led to UTI and how stages of the estrous cycle would impact bacterial colonization in mice. Mice in proestrus, estrus, metestrus, and diestrus were identified by vaginal cytology and inoculated with UPEC in the vaginal tract. Mice were euthanized 1 day after infection, and bacterial loads in the urogenital tract, liver, and spleen were enumerated. Mice in estrus exhibited the highest and most consistent UPEC burdens in all organs, except the bladder. Vaginal inoculation resulted in bladder colonization in a UPEC strain-specific manner. In contrast, transurethral inoculation of UPEC led to bladder colonization. Importantly, inoculation by both routes led to vaginal and uterine colonization and concomitant systemic dissemination to the spleen and liver. The kinetics of bacterial colonization over 2 weeks following vaginal inoculation was comparable in the urogenital tract. Tissue sections revealed the induction of vaginitis and cystitis upon the vaginal instillation of UPEC. In summary, vaginal inoculation of UPEC in mice during estrus represents a novel approach to investigate infection of the kidneys and genital tract and systemic dissemination from the urogenital tract. Our findings suggest that estrogen primes the urogenital tract to create a conducive milieu for UPEC colonization.
登录
查看更多内容
DOI：
10.1038/s41579-020-0324-0
发表时间：
2020-04
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Klein RD;Hultgren SJ
通讯作者：
Hultgren SJ
DOI：
10.1038/nrmicro3432
发表时间：
2015-05
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Flores-Mireles AL;Walker JN;Caparon M;Hultgren SJ
通讯作者：
Hultgren SJ
影响因子：
4.4
作者：
Foxman, Betsy
通讯作者：
Foxman, Betsy
影响因子：
6.4
作者：
Curran, Edward M.;Hart-Van Tassell, Audrey;Nowicki, Stella
通讯作者：
Nowicki, Stella
影响因子：
16.6
作者：
Brannon, John R.;Dunigan, Taryn L.;Hadjifrangiskou, Maria
通讯作者：
Hadjifrangiskou, Maria