Controlling nanostructures of mesoporous silica fibers by supramolecular assembly of genetically modifiable bacteriophages.
Controlling nanostructures of mesoporous silica fibers by supramolecular assembly of genetically modifiable bacteriophages.
复制标题
DOI：
10.1002/anie.201107824
复制
发表时间：
2012-06-25
影响因子：
16.6
通讯作者：
Cao, Binrui
中科院分区：
文献类型：
--
作者：
Mao, Chuanbin;Wang, Fuke;Cao, Binrui
使用阳离子季铵表面活性剂（M41 S家族）[1]、非离子表面活性剂（SBA家族）[2]和阴离子表面活性剂已经成功地制备了有序的介孔二氧化硅。[3]由于其有吸引力的性质，即大的表面积和均匀的孔径，介孔二氧化硅的许多通用应用已被报道在催化，生物吸收，药物递送和纳米反应器。[4]人们普遍认为，有序介孔二氧化硅的形成涉及表面活性剂胶束和硅酸盐的电荷匹配和协同组装成三维晶格结构，如六方，立方或层状。[5]生物颗粒也是形成二氧化硅的良好模板。[6]此外，自然界已经设计了丝状病毒，如M13和fd噬菌体，它们在形态上类似于棒状胶束，但具有比表面活性剂胶束更大的单分散性。此外，它们容易通过自组装形成溶致液晶（LC）相。例如，本研究中使用的半柔性杆状噬菌体（fd或M13）是细菌特异性病毒（长约880 nm，宽约7 nm），可以将其描绘为外壳蛋白沿着环状ssDNA的有序组装（参见支持信息中的图S1）。它们具有高纵横比，并且能够自组装成LC结构。[7]然而，这种噬菌体的高度单分散性和各向异性从未成功地用于合成具有有序孔晶格的介孔二氧化硅。在这里，我们报告的制备有序的介孔二氧化硅纤维与六角形排列的孔（称为ESTA-880）通过使用丝状噬菌体作为模板。此外，我们讨论了成功使用所得的介孔二氧化硅作为制造硫化铅纳米粒子的3D阵列的手段。大自然通过特定蛋白质的自组装创造了令人难以置信的复杂矿物结构，这些蛋白质在环境条件下指导生物矿化。[8]作为最突出的例子之一，硅藻在组装的硅化壳的方向下形成精细的微米/纳米结构的二氧化硅壁。[9]因此，我们也探索噬菌体通过控制其自组装行为来控制二氧化硅纳米结构的能力。由于外源肽可以基因融合到噬菌体的外壳蛋白上产生高度修饰的病毒，[10]而噬菌体的组装行为受到其表面电荷性质的强烈影响，噬菌体本身是控制所得二氧化硅纳米结构的极好的候选模板。[11]通过展示具有不同电荷的肽在丝状噬菌体的侧壁上，我们证明了通过控制噬菌体表面电荷密度和伴随的噬菌体组装行为来成功控制二氧化硅纳米结构。
Well-ordered mesoporous silica has been successfully prepared using cationic quaternary ammonium surfactants (M41S family),[1] nonionic surfactants (SBA family),[2] and anionic surfactants.[3] As a result of their attractive properties, that is, large surface areas and uniform pore sizes, numerous versatile applications of mesoporous silica have been reported in catalysis, bioabsorption, drug delivery, and nanoreactors.[4] It is generally accepted that the formation of wellordered mesoporous silica involves both charge matching and cooperative assembly of surfactant micelles and silicate into 3D lattice structures such as hexagonal, cubic, or lamellar.[5] Biological particles are also good templates for the formation of silica.[6] Moreover, nature has designed filamentous viruses such as M13 and fd bacteriophage that are morphologically similar to rodlike micelles yet possess a greater monodispersity than surfactant micelles. In addition, they readily form a lyotropic liquid-crystalline (LC) phase through self-assembly. As an example, the semiflexible rodlike bacteriophages (fd or M13) used in this study are bacteria-specific viruses (about 880 nm long and about 7 nm wide) that can be pictured as an ordered assembly of coat proteins along circular ssDNA (see Figure S1 in the Supporting Information). They have high aspect ratios and are capable of self-assembling into LC structures.[7] Such a high degree of monodispersity and anisotropy of bacteriophage, however, has never been successfully employed in synthesizing mesoporous silica with ordered pore lattices. Here, we report the preparation of well-ordered mesoporous silica fibers with hexagonally arranged pores (termed OU-880) by use of filamentous bacteriophages as templates. In addition, we discuss the successful use of the resultant mesoporous silica as a means for fabricating 3D arrays of PbS nanoparticles. Nature creates incredibly sophisticated mineral structures through the self-assembly of specific proteins that direct biomineralization at ambient conditions.[8] As one of the most prominent examples, diatoms form fine micro-/nanostructured silica walls under the direction of assembled siliffins.[9] Therefore, we also explore the ability of bacteriophages to control the nanostructures of silica by controlling their self-assembly behavior.Since foreign peptides can be genetically fused to the coat proteins of bacteriophage to produce highly decorated viruses,[10] and the assembly behavior of bacteriophage is strongly affected by its surface charge properties, bacteriophage presents itself as an excellent candidate template for controlling resulting silica nanostructures.[11] By displaying peptides with different charges on the side wall of filamentous bacteriophage, we demonstrate the successful controlling of silica nanostructures through control of the bacteriophage surface charge density and the concomitant bacteriophage assembly behavior.
登录
查看更多内容
影响因子：
64.8
作者：
Cha, JN;Stucky, GD;Deming, TJ
通讯作者：
Deming, TJ
影响因子：
15
作者：
Coleman, NRB;O'Sullivan, N;Holmes, JD
通讯作者：
Holmes, JD
以氨基酸两亲物动态生长纤维为模板自催化溶胶-凝胶协同复制均匀二氧化硅纳米管
影响因子：
3.9
作者：
Lei, Sheng;Zhang, Jing;Huang, Jianbin
通讯作者：
Huang, Jianbin
影响因子：
9.4
作者：
Flynn, CE;Lee, SW;Belcher, AM
通讯作者：
Belcher, AM
影响因子：
16.6
作者：
Mao, Chuanbin;Liu, Aihua;Cao, Binrui
通讯作者：
Cao, Binrui