Dynamics of the methanogenic archaea in tropical estuarine sediments.

Dynamics of the methanogenic archaea in tropical estuarine sediments.
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DOI:
10.1155/2013/582646
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发表时间:
2013
期刊:
Archaea (Vancouver, B.C.)
影响因子:
--
通讯作者:
Varona-Cordero F
Varona-Cordero F
中科院分区:
其他
文献类型:
--
作者:
Torres-Alvarado Mdel R;Fernández FJ;Ramírez Vives F;Varona-Cordero F

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甲烷生成可能代表了海岸泻湖沉积物中厌氧有机质矿化终末阶段的一个关键过程。研究热带滨海泻湖沉积物中产甲烷古菌的时空动态及其与环境变化的关系,以确定环境变化对产甲烷古菌群落的影响。沉积物样品收集在旱季(2月,5月和6月初)和雨季(7月,10月和11月)。微生物分析包括定量的可行的产甲烷古菌(MA)与三个基板和动力学活性的评价,从乙酸在硫酸盐的存在和不存在。评估的环境变量是温度,pH值,Eh,盐度,硫酸盐,固体含量,有机碳,和碳水化合物。MA丰度在雨季(106-107 cells/g)显著高于旱季(104-106 cells/g),甲醇是重要底物。在空间水平上,MA检测到在两个层分析,并没有观察到任何重要的变化,无论是在MA丰度或活动。盐度、硫酸盐、固体、有机碳和Eh是与产甲烷菌群相关的环境变量。提出了一个概念模型来解释的MA的动态。
Methanogenesis may represent a key process in the terminal phases of anaerobic organic matter mineralization in sediments of coastal lagoons. The aim of the present work was to study the temporal and spatial dynamics of methanogenic archaea in sediments of tropical coastal lagoons and their relationship with environmental changes in order to determine how these influence methanogenic community. Sediment samples were collected during the dry (February, May, and early June) and rainy seasons (July, October, and November). Microbiological analysis included the quantification of viable methanogenic archaea (MA) with three substrates and the evaluation of kinetic activity from acetate in the presence and absence of sulfate. The environmental variables assessed were temperature, pH, Eh, salinity, sulfate, solids content, organic carbon, and carbohydrates. MA abundance was significantly higher in the rainy season (106–107 cells/g) compared with the dry season (104–106 cells/g), with methanol as an important substrate. At spatial level, MA were detected in the two layers analyzed, and no important variations were observed either in MA abundance or activity. Salinity, sulfate, solids, organic carbon, and Eh were the environmental variables related to methanogenic community. A conceptual model is proposed to explain the dynamics of the MA.
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