A quantitative study of the division cycle of Caulobacter crescentus stalked cells.
A quantitative study of the division cycle of Caulobacter crescentus stalked cells.
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DOI：
10.1371/journal.pcbi.0040009
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发表时间：
2008-01
影响因子：
4.3
通讯作者：
中科院分区：
文献类型：
--
作者：
细胞通过分裂周期的进展在不同的步骤中受到严格控制，以确保基因组复制和分配到子细胞的完整性。从已发表的实验证据，我们提出了一个控制新月柄杆菌细胞分裂周期的分子机制。的机制，这是基于三个“主调节”蛋白质（CtrA，GcrA，和DnaA）的合成和降解，转换成定量模型，以研究这些和其他细胞周期蛋白质的时间动态。该模型解释了茎柄C.新月形细胞它在野生型细胞中复制蛋白质时间进程，正确模仿许多突变株的表型，并预测目前未知突变体的表型。由于许多参与调控C. crescentus在许多α-变形菌属中是保守的，所提出的机制可能适用于其他在农业和医学上重要的物种。细胞周期是生长中的细胞复制其所有成分并将其或多或少均匀地分配给两个子细胞的事件序列。这些事件的时间和空间组织由非常复杂的基因-蛋白质相互作用网络控制。计算生物学面临的一个挑战是在各种生物体（真核生物和原核生物）中建立这些控制系统的现实，准确，预测的数学模型。为此，我们提出了一个模型的一部分的分子网络控制DNA合成，细胞周期相关的基因表达，DNA甲基化，并在柄细胞的α-变形杆菌新月柄杆菌的细胞分裂。该模型是制定在非线性常微分方程的主要细胞周期调控蛋白在柄杆菌：CtrA，GcrA，DnaA，CcrM，和DivK。动力学速率常数估计，并对野生型和突变细胞的实验观察模型进行测试。该模型被视为一个起点，更全面的模型的未来，将占，此外，为空间不对称的柄杆菌繁殖（群集细胞以及柄细胞），细胞生长和分裂的相关性，和细胞周期检查点。
Progression of a cell through the division cycle is tightly controlled at different steps to ensure the integrity of genome replication and partitioning to daughter cells. From published experimental evidence, we propose a molecular mechanism for control of the cell division cycle in Caulobacter crescentus. The mechanism, which is based on the synthesis and degradation of three “master regulator” proteins (CtrA, GcrA, and DnaA), is converted into a quantitative model, in order to study the temporal dynamics of these and other cell cycle proteins. The model accounts for important details of the physiology, biochemistry, and genetics of cell cycle control in stalked C. crescentus cell. It reproduces protein time courses in wild-type cells, mimics correctly the phenotypes of many mutant strains, and predicts the phenotypes of currently uncharacterized mutants. Since many of the proteins involved in regulating the cell cycle of C. crescentus are conserved among many genera of α-proteobacteria, the proposed mechanism may be applicable to other species of importance in agriculture and medicine. The cell cycle is the sequence of events by which a growing cell replicates all its components and divides them more or less evenly between two daughter cells. The timing and spatial organization of these events are controlled by gene–protein interaction networks of great complexity. A challenge for computational biology is to build realistic, accurate, predictive mathematical models of these control systems in a variety of organisms, both eukaryotes and prokaryotes. To this end, we present a model of a portion of the molecular network controlling DNA synthesis, cell cycle–related gene expression, DNA methylation, and cell division in stalked cells of the α-proteobacterium Caulobacter crescentus. The model is formulated in terms of nonlinear ordinary differential equations for the major cell cycle regulatory proteins in Caulobacter: CtrA, GcrA, DnaA, CcrM, and DivK. Kinetic rate constants are estimated, and the model is tested against available experimental observations on wild-type and mutant cells. The model is viewed as a starting point for more comprehensive models of the future that will account, in addition, for the spatial asymmetry of Caulobacter reproduction (swarmer cells as well as stalked cells), the correlation of cell growth and division, and cell cycle checkpoints.
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DOI：
10.1073/pnas.86.1.119
发表时间：
1989-01-01
影响因子：
11.1
作者：
DINGWALL, A;SHAPIRO, L
通讯作者：
SHAPIRO, L
影响因子：
64.5
作者：
Domian, IJ;Quon, KC;Shapiro, L
通讯作者：
Shapiro, L
影响因子：
11.4
作者：
Collier, J;Murray, SR;Shapiro, L
通讯作者：
Shapiro, L
DOI：
10.1073/pnas.071538098
发表时间：
2001-04-10
影响因子：
11.1
作者：
Grünenfelder, B;Rummel, G;Jenal, U
通讯作者：
Jenal, U
影响因子：
3.3
作者：
Cross, FR;Schroeder, L;Chen, KC
通讯作者：
Chen, KC