Tomato Chlorosis Virus Infection Facilitates Bemisia tabaci MED Reproduction by Elevating Vitellogenin Expression.
Tomato Chlorosis Virus Infection Facilitates Bemisia tabaci MED Reproduction by Elevating Vitellogenin Expression.
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番茄褪绿病毒感染通过提高卵黄蛋白原表达促进烟粉虱 MED 繁殖。
DOI：
10.3390/insects12020101
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发表时间：
2021-01-25
期刊：
影响因子：
3
通讯作者：
Zhou X
中科院分区：
文献类型：
--
作者：
Huang L;Shi X;Shi J;Zhang Z;Fang Y;Zhang Z;Pan Q;Zheng L;Gao Y;Zhang D;Tan X;Liu Y;Zhou X
甘薯粉虱是一种多食性、全球性的入侵害虫。它可以通过取食直接危害蔬菜和农作物，也可以通过传播植物病毒间接危害蔬菜和农作物。以前，我们表明，病毒感染的寄主植物可以促进B。烟粉虱MED（Q型）繁殖。在这里，使用粉虱-番茄褪绿病毒（ToCV）-番茄系统，我们研究了ToCV如何调节B。烟粉虱繁殖，以促进其传播。ToCV感染显著增加烟粉虱的繁殖力和卵黄蛋白原基因（Vg）的相对表达量。无论是否感染ToCV，Vg表达沉默均能抑制粉虱的卵巢发育和繁殖力。这些综合结果表明ToCV感染增加了B。通过提高卵黄蛋白原基因表达提高烟粉虱MED繁殖力。植物病原病毒的传播主要依靠昆虫媒介。植物病毒可以通过调节载体来增强其传播。以前，我们表明，在病毒感染的植物上饲养可以促进甘薯粉虱，烟粉虱MED（Q生物型）的繁殖。在这项研究中，使用粉虱-番茄褪绿病毒（ToCV）-番茄系统，我们研究了ToCV如何调节B。烟粉虱MED繁殖，以促进其传播。在这里，我们假设ToCV感染的番茄植株会增加B。通过提高卵黄蛋白原（Vg）基因的表达，烟粉虱MED繁殖力。结果表明，该菌的繁殖力和B的相对表达量均高于对照。在第4、8、12、16、20和24天对ToCV感染和未感染的番茄植物测量烟粉虱MED Vg。Vg对B的作用。在ToCV存在和不存在的情况下，使用饮食RNAi检查烟粉虱MED繁殖。ToCV感染显著增加了B。在第12、16和20天，烟粉虱MED繁殖力，并且在第8、12和16天增加Vg表达。B的卵巢发育和生殖力。当Vg在ToCV感染或不感染的情况下被沉默时，烟粉虱MED被抑制。这些综合结果表明ToCV感染增加了B。提高Vg的表达，提高烟粉虱MED的繁殖力。
The sweet potato whitefly, Bemisia tabaci, is a polyphagous, global invasive insect pest. It can damage vegetables and crops directly by feeding and indirectly by transmitting plant viruses. Previously, we showed that virus infection of host plants can promote B. tabaci MED (Q biotype) reproduction. Here, using a whitefly-tomato chlorosis virus (ToCV)-tomato system, we investigated how ToCV modulates B. tabaci reproduction to facilitate its spread. ToCV infection significantly increased whitefly fecundity and the relative expression of vitellogenin gene (Vg). Both ovarian development and fecundity of whitefly were suppressed when Vg expression was silenced with or without ToCV infection. These combined results reveal that ToCV infection increases B. tabaci MED fecundity via elevated vitellogenin gene expression. Transmission of plant pathogenic viruses mostly relies on insect vectors. Plant virus could enhance its transmission by modulating the vector. Previously, we showed that feeding on virus infected plants can promote the reproduction of the sweet potato whitefly, Bemisia tabaci MED (Q biotype). In this study, using a whitefly-Tomato chlorosis virus (ToCV)-tomato system, we investigated how ToCV modulates B. tabaci MED reproduction to facilitate its spread. Here, we hypothesized that ToCV-infected tomato plants would increase B. tabaci MED fecundity via elevated vitellogenin (Vg) gene expression. As a result, fecundity and the relative expression of B. tabaci MED Vg was measured on ToCV-infected and uninfected tomato plants on days 4, 8, 12, 16, 20 and 24. The role of Vg on B. tabaci MED reproduction was examined in the presence and absence of ToCV using dietary RNAi. ToCV infection significantly increased B. tabaci MED fecundity on days 12, 16 and 20, and elevated Vg expression on days 8, 12 and 16. Both ovarian development and fecundity of B. tabaci MED were suppressed when Vg was silenced with or without ToCV infection. These combined results suggest that ToCV infection increases B. tabaci MED fecundity via elevated Vg expression.
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影响因子：
4.4
作者：
Kaur N;Chen W;Zheng Y;Hasegawa DK;Ling KS;Fei Z;Wintermantel WM
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影响因子：
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作者：
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