Transcription Factors Drive Opposite Relationships between Gene Age and Tissue Specificity in Male and Female Drosophila Gonads.
Transcription Factors Drive Opposite Relationships between Gene Age and Tissue Specificity in Male and Female Drosophila Gonads.
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DOI：
10.1093/molbev/msab011
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发表时间：
2021-05-04
影响因子：
10.7
通讯作者：
Zhao L
中科院分区：
文献类型：
--
作者：
Witt E;Svetec N;Benjamin S;Zhao L
进化上年轻的基因通常在不同物种的睾丸中优先表达。虽然众所周知，年长的基因通常比年轻的基因表达得更广泛，但形成这种模式的属性尚不清楚。较老的基因可能会在其他组织中均匀地表达，或者在某些组织中比其他组织表达得更快。利用果蝇的基因表达数据，我们证实了之前的发现，即年轻的基因不成比例地偏向睾丸，而年长的基因不成比例地偏向卵巢。我们发现，基因年龄与基因表达之间的关系在卵巢中比其他任何组织都强，在睾丸中最弱。我们在果蝇睾丸上进行了ATAC-SEQ，发现尽管所有年龄段的基因在睾丸中比在卵巢中更有可能具有开放的启动子染色质，但单靠启动子染色质并不能解释较老基因对卵巢的偏见。相反，我们发现上游转录因子(TF)的表达可以很好地预测基因在卵巢中的表达，而不是在睾丸中的表达。在卵巢中，转录因子的表达比开放启动子染色质更能预测基因的表达，而睾丸基因的表达同样受到转录因子表达和开放启动子染色质的影响。我们认为，睾丸唯一能够表达由相对较少的TF控制的年轻基因，而具有更多TF伙伴的较老基因广泛表达，最可能在卵巢表达。睾丸允许广泛的基线表达，对调节变化相对不敏感，而卵巢转录组对反式调节更敏感，具有更高的基因表达上限。
Evolutionarily young genes are usually preferentially expressed in the testis across species. Although it is known that older genes are generally more broadly expressed than younger genes, the properties that shaped this pattern are unknown. Older genes may gain expression across other tissues uniformly, or faster in certain tissues than others. Using Drosophila gene expression data, we confirmed previous findings that younger genes are disproportionately testis biased and older genes are disproportionately ovary biased. We found that the relationship between gene age and expression is stronger in the ovary than any other tissue and weakest in testis. We performed ATAC-seq on Drosophila testis and found that although genes of all ages are more likely to have open promoter chromatin in testis than in ovary, promoter chromatin alone does not explain the ovary bias of older genes. Instead, we found that upstream transcription factor (TF) expression is highly predictive of gene expression in ovary but not in testis. In the ovary, TF expression is more predictive of gene expression than open promoter chromatin, whereas testis gene expression is similarly influenced by both TF expression and open promoter chromatin. We propose that the testis is uniquely able to express younger genes controlled by relatively few TFs, whereas older genes with more TF partners are broadly expressed with peak expression most likely in the ovary. The testis allows widespread baseline expression that is relatively unresponsive to regulatory changes, whereas the ovary transcriptome is more responsive to trans-regulation and has a higher ceiling for gene expression.
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影响因子：
7
作者：
Trizzino M;Park Y;Holsbach-Beltrame M;Aracena K;Mika K;Caliskan M;Perry GH;Lynch VJ;Brown CD
通讯作者：
Brown CD
影响因子：
4.5
作者：
Ding Y;Zhao L;Yang S;Jiang Y;Chen Y;Zhao R;Zhang Y;Zhang G;Dong Y;Yu H;Zhou Q;Wang W
通讯作者：
Wang W
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
14.9
作者：
Leader DP;Krause SA;Pandit A;Davies SA;Dow JAT
通讯作者：
Dow JAT
DOI：
10.1073/pnas.0509809103
发表时间：
2006-06-27
影响因子：
11.1
作者：
Levine, Mia T.;Jones, Corbin D.;Begun, David J.
通讯作者：
Begun, David J.