Bacterial diets differentially alter lifespan and healthspan trajectories in C. elegans.
Bacterial diets differentially alter lifespan and healthspan trajectories in C. elegans.
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DOI：
10.1038/s42003-020-01379-1
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发表时间：
2020-11-06
影响因子：
5.9
通讯作者：
Curran SP
中科院分区：
文献类型：
--
作者：
Stuhr NL;Curran SP
饮食是生活中变化较大的方面之一，因为生物体在其自然栖息地中暴露于各种选择。在实验室中，C. elegans是在细菌单一培养物上培养的，传统上E.大肠杆菌B菌株OP 50和自发产生的微生物污染物被去除，以限制实验的可变性，因为饮食-包括污染物的存在-可以对动物的生理产生强有力的影响。为了使菜单多样化，文化C。在实验室中，我们分离并培养了三种这样的微生物：甲基杆菌、黄单胞菌和鞘氨醇单胞菌。这些细菌食物的营养成分是独特的，当喂给C。elegans可以差异性地改变多种生活史特征，包括发育、繁殖和代谢。鉴于每种食物来源对特定生理属性的影响，我们全面评估了这些细菌对动物健康的影响，并设计了在生命周期内利用不同食物组合的蓝图，以促进长寿。在实验室中使用的细菌食物选择的扩展将提供一个关键的工具，以更好地了解细菌饮食的复杂性以及随后的生理和基因表达变化。施图尔和柯伦描绘了C. elegans在六种细菌饮食下：三种最常用的E.大肠杆菌菌株OP 50、HT 115、HB 101沿着甲基杆菌属、黄单胞菌属和鞘氨醇单胞菌属。这些结果显示了饮食如何不同地改变多种生活史特征，包括发育，繁殖和代谢，并可以更好地了解细菌饮食的复杂性以及随后的生理学和基因表达变化。
Diet is one of the more variable aspects in life due to the variety of options that organisms are exposed to in their natural habitats. In the laboratory, C. elegans are raised on bacterial monocultures, traditionally the E. coli B strain OP50, and spontaneously occurring microbial contaminants are removed to limit experimental variability because diet—including the presence of contaminants—can exert a potent influence over animal physiology. In order to diversify the menu available to culture C. elegans in the lab, we have isolated and cultured three such microbes: Methylobacterium, Xanthomonas, and Sphingomonas. The nutritional composition of these bacterial foods is unique, and when fed to C. elegans, can differentially alter multiple life history traits including development, reproduction, and metabolism. In light of the influence each food source has on specific physiological attributes, we comprehensively assessed the impact of these bacteria on animal health and devised a blueprint for utilizing different food combinations over the lifespan, in order to promote longevity. The expansion of the bacterial food options to use in the laboratory will provide a critical tool to better understand the complexities of bacterial diets and subsequent changes in physiology and gene expression. Stuhr and Curran profile the metabolic, lifespan and health outcomes of C. elegans under six bacterial diets: the three most commonly used E. coli strains OP50, HT115, HB101, along with Methylobacterium, Xanthomonas, and Sphingomonas. These results show how diet can differentially alter multiple life history traits including development, reproduction, and metabolism, and can provide a better understanding of the complexities of bacterial diets and subsequent changes in physiology and gene expression.
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