28-Day hindlimb unweighting reduces expression of Rho kinase and inhibits its effects in femoral artery of rat
28-Day hindlimb unweighting reduces expression of Rho kinase and inhibits its effects in femoral artery of rat
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28天后肢减重降低Rho激酶表达并抑制其在大鼠股动脉中的作用
DOI：
10.1007/s13105-015-0398-8
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发表时间：
2015-03
影响因子：
3.4
通讯作者：
Ma, Jin
中科院分区：
文献类型：
--
作者：
Bao, Jun-Xiang;Ren, Xin-Ling;Ma, Hong-Zhe;Ma, Jin
以往的研究表明，Rho激酶（ROCK）在后肢减重（HU）引起的大鼠后肢血管收缩减少中的作用不一致。本研究旨在确定ROCK在HU诱导的股动脉血管收缩减少的介导中的作用。28-采用日龄HU大鼠作为动物模型。用ROCK抑制剂Y-27632或不加ROCK抑制剂Y-27632测定股动脉等长收缩力。还检测了ROCK的表达及其对下游靶点的影响。结果表明：（1）HU可显著降低苯肾上腺素（PE）和氯化钾（KCl）诱发的股动脉收缩反应（P< 0.05），证实了前人的研究结果。(2)用Y-27632抑制ROCK，在CON和HU中产生相等的血管收缩减少。(3)HU可显著降低ROCK Ⅱ的表达及ROCK对肌球蛋白轻链磷酸酶（MLCP）和MLC的影响（P< 0.05），增加p65核转位（P< 0.05）和诱导型一氧化氮合酶（iNOS）的表达（P< 0.05）。(4)HU能显著增加股动脉NO生成（P< 0.05），Y-27632能显著增强HU的这种作用（P< 0.01）。这些发现表明，28天HU降低了ROCK对与股动脉血管收缩直接相关（MLCP和MLC）和可能间接相关（NF-κB/iNOS/NO通路）的下游靶点的表达和作用。
Previous studies have demonstrated inconsistent roles of Rho kinase (ROCK) in the decreased vasoconstriction of rat hindquarter vessels induced by hindlimb unweighting (HU). The present study was designed to determine the unclear role of ROCK in the mediation of HU-induced decreased femoral arterial vasoconstriction. 28-day HU rat was adopted as the animal model. With or without Y-27632, a ROCK inhibitor, isometric force of femoral artery was measured. The expression of ROCK and its effects on downstream targets were also examined. Results showed that (1) HU caused a significant decrease of the phenylephrine (PE)-evoked and potassium chloride (KCl)-evoked femoral arterial vasoconstriction (P< 0.05), confirming the functional findings by previous studies. (2) Inhibition of ROCK with Y-27632 produced an equal reduction of the vasoconstriction in CON and HU. (3) HU significantly decreased ROCK II expression and the effects of ROCK on myosin light-chain phosphatase (MLCP) and MLC (P< 0.05), but increased p65 nuclear translocation (P< 0.05) and inducible nitric oxide synthase (iNOS) expression (P< 0.05). (4) HU significantly (P< 0.05) increased NO production in femoral arteries, with Y-27632 significantly (P< 0.01) amplifying this effect. These findings have revealed that 28-day HU reduced the expression and effects of ROCK on downstream targets both directly (MLCP and MLC) and possibly indirectly (NF-κB/iNOS/NO pathway) related to vasoconstriction in femoral arteries.
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DOI：
--
发表时间：
2004-10
期刊：
Hang tian yi xue yu yi xue gong cheng = Space medicine & medical engineering
影响因子：
--
作者：
Zhi-li Li;Ming Yuan;Shi-zhong Jiang;De-sheng Wang;Hui-juan Wang
通讯作者：
Zhi-li Li;Ming Yuan;Shi-zhong Jiang;De-sheng Wang;Hui-juan Wang
失重环境下人工重力的区域特异性血管重塑及其预防
影响因子：
3
作者：
张立藩
通讯作者：
张立藩
DOI：
--
发表时间：
1996-09
期刊：
Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology
影响因子：
--
作者：
Jin Ma;Li-fan Zhang;Zhi-Bin Yu
通讯作者：
Jin Ma;Li-fan Zhang;Zhi-Bin Yu
影响因子：
3.3
作者：
R. Purdy;S. Duckles;D. Krause;K. M. Rubera;D. Sara
通讯作者：
R. Purdy;S. Duckles;D. Krause;K. M. Rubera;D. Sara
模拟微重力过程中大鼠大脑和后肢动脉钾通道的差异激活
DOI：
10.1152/ajpheart.00143.2004
发表时间：
2004-10-01
影响因子：
4.8
作者：
Fu, ZJ;Xie, MJ;Ma, J
通讯作者：
Ma, J