Predicting severity of cartilage damage in a post-traumatic porcine model: Synovial fluid and gait in a support vector machine.
Predicting severity of cartilage damage in a post-traumatic porcine model: Synovial fluid and gait in a support vector machine.
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DOI：
10.1371/journal.pone.0268198
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
对关节损伤的炎症反应被认为在骨关节炎的发展中起关键作用。在这项临床前研究中，我们假设滑液中存在炎性细胞因子，以及受伤腿上的负荷改变，与ACL损伤后宏观软骨损伤的更大发展相关。36只尤卡坦小型猪接受ACL横断，并随机分配至：1）无进一步治疗，2）ACL重建，或3）支架增强ACL修复。术前和术后多个时间点采集滑液样本和步态数据。使用多重测定法测量细胞因子水平。在第52周人道处死后进行肉眼软骨评估。一般估计方程模型发现滑液中IL-1α、IL-1 RA、IL-2、IL-4、IL-6和IL-10以及MMP-2、MMP-3、MMP-12和MMP-13的存在与更好的软骨结局相关。手术后腿和对侧后腿的峰值压力较高，软骨结果较差。用滑液和步态指标构建的支持向量机也证明了细胞因子的存在可以预测更好的软骨结果。总之，这项临床前分析表明，缺乏细胞因子的滑液可能是关节损伤后软骨更容易发生病理性变化的一个可能指标。
The inflammatory response to joint injury has been thought to play a key role in the development of osteoarthritis. In this preclinical study, we hypothesized that synovial fluid presence of inflammatory cytokines, as well as altered loading on the injured leg, would be associated with greater development of macroscopic cartilage damage after an ACL injury. Thirty-six Yucatan minipigs underwent ACL transection and were randomized to: 1) no further treatment, 2) ACL reconstruction, or 3) scaffold-enhanced ACL restoration. Synovial fluid samples and gait data were obtained pre-operatively and at multiple time points post-operatively. Cytokine levels were measured using a multiplex assay. Macroscopic cartilage assessments were performed following euthanasia at 52 weeks. General estimating equation modeling found the presence of IL-1α, IL-1RA, IL-2, IL-4, IL-6, and IL-10 and MMP-2, MMP-3, MMP-12, and MMP-13 in the synovial fluid was associated with better cartilage outcomes. Higher peak pressure for the surgical hind leg and contralateral hind leg aligned with worse cartilage outcomes. A support vector machine built with synovial fluid and gait metrics also demonstrated cytokine presence was predictive of better cartilage outcomes. In conclusion, this preclinical analysis suggests that synovial fluid devoid of cytokines may be a possible indicator that cartilage is more at risk of becoming pathologic after joint injury.
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DOI：
10.1177/0363546521989265
发表时间：
2021-03
期刊：
The American journal of sports medicine
影响因子：
--
作者：
Karamchedu NP;Murray MM;Sieker JT;Proffen BL;Portilla G;Costa MQ;Molino J;Fleming BC
通讯作者：
Fleming BC
影响因子：
2.3
作者：
Heard BJ;Martin L;Rattner JB;Frank CB;Hart DA;Krawetz R
通讯作者：
Krawetz R
影响因子：
4.9
作者：
Haraden, Collin A.;Huebner, Janet L.;Kraus, Virginia Byers
通讯作者：
Kraus, Virginia Byers
影响因子：
7
作者：
Neuman, P.;Dahlberg, L. E.;Struglics, A.
通讯作者：
Struglics, A.
影响因子：
5.1
作者：
Attur M;Krasnokutsky-Samuels S;Samuels J;Abramson SB
通讯作者：
Abramson SB