Convergent evolution of pathogenicity islands in helper cos phage interference.
Convergent evolution of pathogenicity islands in helper cos phage interference.
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DOI：
10.1098/rstb.2015.0505
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发表时间：
2016-11-05
期刊：
影响因子：
--
通讯作者：
Penadés JR
中科院分区：
文献类型：
--
作者：
Carpena N;Manning KA;Dokland T;Marina A;Penadés JR
金黄色葡萄球菌致病岛（SaPI）是噬菌体卫星，利用其辅助细菌的生命周期为自己的利益。大多数SaPI都是由它们的辅助服务器使用一个headful（pac）打包机制打包的。这些SaPI通过多种策略干扰pac噬菌体繁殖，包括重定向噬菌体衣壳组装以形成小衣壳，这是一个依赖于SaPI编码的cpmA和cpmB基因表达的过程。另一个SaPI亚家族由cos型β-内酰胺酶诱导和包装，尽管这些cos SaPI也阻断了其诱导β-内酰胺酶的生命周期，但这种干扰机制的基础仍有待破译。在这里，我们已经确定并表征了SaPI干扰cos噬菌体繁殖的一种机制。这种机制依赖于一个SAPI编码的基因，CCM，它编码的蛋白质参与生产的小等距衣壳，相比长形的辅助噬菌体衣壳。由于Ccm和CpmAB蛋白质在序列上完全不相关，这种策略代表了趋同进化的一个迷人的例子。此外，该结果还表明，产生SaPI大小的颗粒是SaPI进化过程中保守的噬菌体干扰的广泛策略。这篇文章是主题问题“新细菌学”的一部分。
Staphylococcus aureus pathogenicity islands (SaPIs) are phage satellites that exploit the life cycle of their helper phages for their own benefit. Most SaPIs are packaged by their helper phages using a headful (pac) packaging mechanism. These SaPIs interfere with pac phage reproduction through a variety of strategies, including the redirection of phage capsid assembly to form small capsids, a process that depends on the expression of the SaPI-encoded cpmA and cpmB genes. Another SaPI subfamily is induced and packaged by cos-type phages, and although these cos SaPIs also block the life cycle of their inducing phages, the basis for this mechanism of interference remains to be deciphered. Here we have identified and characterized one mechanism by which the SaPIs interfere with cos phage reproduction. This mechanism depends on a SaPI-encoded gene, ccm, which encodes a protein involved in the production of small isometric capsids, compared with the prolate helper phage capsids. As the Ccm and CpmAB proteins are completely unrelated in sequence, this strategy represents a fascinating example of convergent evolution. Moreover, this result also indicates that the production of SaPI-sized particles is a widespread strategy of phage interference conserved during SaPI evolution. This article is part of the themed issue ‘The new bacteriology’.
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影响因子：
14.9
作者：
Ferrer MD;Quiles-Puchalt N;Harwich MD;Tormo-Más MÁ;Campoy S;Barbé J;Lasa I;Novick RP;Christie GE;Penadés JR
通讯作者：
Penadés JR
影响因子：
3.6
作者：
Mir-Sanchis, Ignacio;Martinez-Rubio, Roser;Penades, Jose R.
通讯作者：
Penades, Jose R.
影响因子：
14.9
作者：
Quiles-Puchalt N;Tormo-Más MÁ;Campoy S;Toledo-Arana A;Monedero V;Lasa I;Novick RP;Christie GE;Penadés JR
通讯作者：
Penadés JR
影响因子：
14.8
作者：
Kelley LA;Mezulis S;Yates CM;Wass MN;Sternberg MJ
通讯作者：
Sternberg MJ
影响因子：
3.7
作者：
Konagurthu, Arun S.;Reboul, Cyril F.;Buckle, Ashley M.
通讯作者：
Buckle, Ashley M.