Detection of clonal T-cell receptor gamma-chain gene rearrangements in Reed-Sternberg cells of classic Hodgkin disease.
Detection of clonal T-cell receptor gamma-chain gene rearrangements in Reed-Sternberg cells of classic Hodgkin disease.
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经典霍奇金病 Reed-Sternberg 细胞中克隆 T 细胞受体 γ 链基因重排的检测。
作者：
V. Seitz;M. Hummel;Theresa Marafioti;I. Anagnostopoulos;C. Assaf;H. Stein
最近的分子单细胞研究表明，在大约95%的情况下，经典霍奇金病（HD）的Reed-Sternberg细胞来源于生发中心起源的B细胞。迄今为止，试图确定其余病例的细胞性质的努力都失败了。为了阐明它们是否来源于T细胞，本研究通过单拷贝聚合酶链反应检测了来自13例T细胞标志物阳性病例和6例空细胞表型病例的791个单个CD 30（+）Hodgkin和Reed-Sternberg（HRS）细胞的重排T细胞受体-γ（TCR-γ）基因。13例HRS细胞阳性的经典HD病例中，2例可检测到单克隆重排的TCR-γ基因，而在空细胞病例中未检测到。8例T细胞标记阳性病例和所有6例空细胞病例也进行了免疫球蛋白基因重排研究。8例T细胞标记阳性病例中有6例存在克隆性免疫球蛋白基因重排。2例无重排免疫球蛋白基因的病例是那些含有克隆性TCR-γ重排且缺乏B细胞特异性激活蛋白表达的病例。从这些发现中，我们得出结论，经典HD与T细胞衍生的HRS细胞的情况下肯定存在，虽然他们的总发病率为1%至2%是非常低的。即使在T细胞标记阳性的病例中，也只有少数（15%）来自T细胞。大多数（85%）起源于B细胞，表明HRS细胞表达的T细胞抗原与非霍奇金淋巴瘤中表达的T细胞抗原相反，不是谱系特异性的。
Recent molecular single-cell studies have shown that in approximately 95% of cases, Reed-Sternberg cells of classic Hodgkin disease (HD) are derived from B cells of germinal center origin. Attempts to determine the cellular nature of the remaining cases have so far failed. To clarify whether they are derived from T cells, this study examined 791 single CD30(+) Hodgkin and Reed-Sternberg (HRS) cells from 13 T-cell marker-positive cases and from 6 cases with null-cell phenotype for rearranged T-cell receptor-gamma (TCR-gamma) genes by single copy polymerase chain reaction. Monoclonally rearranged TCR-gamma genes were detectable in 2 of the 13 classic HD cases with T-cell marker-positive HRS cells, with none detectable in the null-cell cases. Eight of the T-cell marker-positive cases and all 6 null-cell cases were also studied for rearrangements of immunoglobulin genes. Six of the 8 T-cell marker-positive cases harbored clonal immunoglobulin gene rearrangements. The 2 cases without rearranged immunoglobulin genes were those that contained clonal TCR-gamma rearrangements and lacked expression of the B-cell-specific activator protein. From these findings we conclude that cases of classic HD with T-cell-derived HRS cells definitely exist, although their overall incidence at 1% to 2% is very low. Even within the T-cell marker-positive cases only a minority (15%) were derived from T cells. The majority (85%) originated from B cells, indicating that the T-cell antigens expressed by HRS cells are, in contrast to those expressed in non-Hodgkin lymphoma, not lineage specific.
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霍奇金病中的免疫球蛋白基因重排。
影响因子：
3.3
作者：
Weiss,LM;Strickler,JG;Hu,E;Warnke,RA;Sklar,J
通讯作者：
Sklar,J
影响因子：
158.5
作者：
DAVIS, TH;MORTON, CC;KADIN, ME
通讯作者：
KADIN, ME
基因组 DNA 扩增表明间变性大细胞淋巴瘤中存在 t(2;5) (p23;q35)，但其他非霍奇金淋巴瘤、霍奇金病或淋巴瘤样丘疹病中不存在。
影响因子：
20.3
作者：
Sarris,AH;Luthra,R;Papadimitracopoulou,V;Waasdorp,M;Dimopoulos,MA;McBride,JA;Cabanillas,F;Duvic,M;Deisseroth,A;Morris,SW;Pugh,WC
通讯作者：
Pugh,WC
影响因子：
158.5
作者：
WEISS, LM;MOVAHED, LA;SKLAR, J
通讯作者：
SKLAR, J
霍奇金病的免疫球蛋白和 T 细胞受体 β 链基因重排分析：对谱系测定和鉴别诊断的影响。
DOI：
10.1073/pnas.83.20.7942
发表时间：
1986
影响因子：
11.1
作者：
Knowles2nd,DM;Neri,A;Pelicci,PG;Burke,JS;Wu,A;Winberg,CD;Sheibani,K;Dalla-Favera,R
通讯作者：
Dalla-Favera,R