The P-Type ATPase PA1429 Regulates Quorum-Sensing Systems and Bacterial Virulence.
The P-Type ATPase PA1429 Regulates Quorum-Sensing Systems and Bacterial Virulence.
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P 型 ATP 酶 PA1429 调节群体感应系统和细菌毒力
DOI：
10.3389/fmicb.2017.02449
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发表时间：
2017
影响因子：
5.2
通讯作者：
Liang H
中科院分区：
文献类型：
--
作者：
Zhang Y;Qin J;Tan B;Kong W;Chen G;Zhang C;Liang H
铜绿假单胞菌正在成为一种日益流行的病原体，能够在免疫功能低下的患者中引起许多危及生命的感染。三个分层排列的群体感应（QS）系统，即las、rhl和pqs在协调铜绿假单胞菌中的毒力表达中起关键作用。然而，pqs系统的调节机制尚未完全阐明。为了鉴定参与合成假单胞菌喹诺酮信号（PQS）的新基因，构建了转座子诱变文库。PA1429对PQS的生物合成有抑制作用。PA1429缺失突变体在小鼠急性肺部感染模型中也表现出增加的细菌运动性、生物膜形成和毒力。有趣的是，它还显示出对氧化应激的耐受性降低。PA1429缺失背景下的突变pqsH恢复了细菌对H2O2的敏感性。此外，我们的数据表明，PA 1429抑制las和rhl系统的表达。总之，这些结果为铜绿假单胞菌群体感应和毒力表达的复杂调控网络提供了新的见解。
Pseudomonas aeruginosa is becoming an increasingly prevalent pathogen, capable of causing numerous life threatening infections in immunocompromised patients. The three hierarchically arranged quorum sensing (QS) systems, namely las, rhl, and pqs play key roles in coordinating virulence expression in P. aeruginosa. However, the regulatory mechanisms of the pqs system have not been fully elucidated. To identify new genes involved in synthesis of the Pseudomonas quinolone signal (PQS), a transposon mutagenesis library was constructed. PA1429 was found to inhibit PQS biosynthesis. The PA1429 deletion mutant also exhibited increased bacterial motility, biofilm formation, and virulence in a mouse model of acute lung infection. Interestingly, it also displayed reduced tolerance to oxidative stress. Mutated pqsH in the PA1429 deletion background restored bacterial susceptibility to H2O2. In addition, our data showed that PA1429 repressed the expression of las and rhl systems. Overall, these results provide new insights into the complex regulatory networks of quorum-sensing and virulence expression in P. aeruginosa.
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影响因子：
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