Identification and Expression Profile Analysis of the OSCA Gene Family Related to Abiotic and Biotic Stress Response in Cucumber.
Identification and Expression Profile Analysis of the OSCA Gene Family Related to Abiotic and Biotic Stress Response in Cucumber.
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黄瓜非生物和生物胁迫反应相关 OSCA 基因家族的鉴定及表达谱分析
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简单总结 高渗透压门控钙渗透通道 (OSCA) 是参与多种生物过程的钙非选择性阳离子通道蛋白。本工作对黄瓜OSCA家族成员进行了系统分析，包括其序列特征、系统发育关系、保守基序、基因结构、启动子区域和组织表达模式。此外，还确定了不同渗透相关的非生物胁迫[盐（NaCl）、干旱（PEG）和脱落酸（ABA）]和三种生物胁迫[白粉病（PM）、霜霉病（DM）和根结线虫（RKN）]对OSCA家族基因的影响。结果表明，黄瓜 OSCA 基因在响应渗透相关的非生物胁迫和病原体入侵方面发挥着重要作用。总体而言，本研究为黄瓜OSCA家族基因的生物学功能和进化过程的研究奠定了基础。摘要 钙离子是重要的第二信使，在信号转导途径中发挥着重要作用。高渗透压门控钙渗透通道 (OSCA) 基因家族成员在响应渗透相关的非生物胁迫以及其他非生物和生物胁迫方面发挥着关键的调节作用，这在拟南芥、水稻、玉米和小麦等许多植物物种中都有报道。然而，目前尚未见关于黄瓜OSCA基因的鉴定及表达谱的报道。本研究共鉴定出9个OSCA基因，这些基因在黄瓜的6条染色体上分布不均匀。系统发育分析表明，黄瓜、拟南芥、水稻和玉米的OSCA聚类为4个进化枝。 CsOSCA的基序排列高度保守，各组的CsOSCA基因具有相似的遗传结构。在CsOSCA基因的启动子区域中分别鉴定出11种和10种与激素和应激相关的顺式元件。基因表达分析表明，CsOSCA基因在不同组织中具有不同的表达模式，其中一些基因受到三种渗透相关的非生物胁迫（盐、干旱和ABA）和三种生物胁迫（白粉病、霜霉病和根结线虫）的调节。该研究作为黄瓜OSCA基因家族的首次全基因组鉴定和表征，为研究该基因家族的生物学功能和进化过程奠定了基础，对于开发抗逆黄瓜品种具有重要意义。
Simple Summary Hyperosmolality-gated calcium-permeable channels (OSCAs) are calcium nonselective cation channel proteins involved in multiple biological processes. In this work, the members of the OSCA family in cucumber were systematically analyzed, including their sequence characteristics, phylogenetic relationships, conserved motifs, gene structures, promoter regions, and tissue expression patterns. In addition, the effects of different osmotic-related abiotic stresses [salt (NaCl), drought (PEG), and abscisic acid (ABA)] and three biotic stresses [powdery mildew (PM), downy mildew (DM), and root-knot nematode (RKN)] on OSCA family genes were also determined. The results indicated that cucumber OSCA genes play important roles in response to osmotic-related abiotic stresses and pathogen invasion. Overall, this study lays a foundation for research on the biological function and evolutionary process of OSCA family genes in cucumber. Abstract Calcium ions are important second messengers, playing an important role in the signal transduction pathways. Hyperosmolality gated calcium-permeable channels (OSCA) gene family members play critical modulating roles in response to osmotic-related abiotic stress as well as other abiotic and biotic stresses, which has been reported in many plant species such as Arabidopsis, rice, maize, and wheat. However, there has been no report about the identification and expression profile of the OSCA genes in cucumber. In this study, a total of nine OSCA genes were identified, which are unevenly distributed on the six chromosomes of cucumber. Phylogenetic analysis revealed that the OSCAs of cucumber, Arabidopsis, rice and maize were clustered into four clades. The motif arrangement of CsOSCAs was strongly conserved, and the CsOSCA genes in each group had similar genetic structure. A total of 11 and 10 types of cis-elements related to hormone and stress, respectively, were identified in the promoter regions of CsOSCA genes. Gene expression analysis indicated that the CsOSCA genes have different expression patterns in various tissues, and some of them were regulated by three osmotic-related abiotic stresses (salt, drought and ABA) and three biotic stresses (powdery mildew, downy mildew, and root-knot nematode). As the first genome-wide identification and characterization of the OSCA gene family in cucumber, this study lays a foundation for research on the biological function and evolutionary process of this gene family, which is of great significance for exploiting stress resistant cucumber varieties.
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钙渗透通道OSCA1.3调节植物的气孔免疫。
影响因子：
64.8
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Thor K;Jiang S;Michard E;George J;Scherzer S;Huang S;Dindas J;Derbyshire P;Leitão N;DeFalco TA;Köster P;Hunter K;Kimura S;Gronnier J;Stransfeld L;Kadota Y;Bücherl CA;Charpentier M;Wrzaczek M;MacLean D;Oldroyd GED;Menke FLH;Roelfsema MRG;Hedrich R;Feijó J;Zipfel C
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Zipfel C
影响因子：
1.2
作者：
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影响因子：
7.4
作者：
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通讯作者：
Davies, Julia M.
影响因子：
4.2
作者：
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通讯作者：
Pei, Zhen-Ming
影响因子：
4.4
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Lin K