Identification and evaluation of anti-inflammatory properties of aqueous components extracted from sesame (Sesamum indicum) oil.
Identification and evaluation of anti-inflammatory properties of aqueous components extracted from sesame (Sesamum indicum) oil.
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DOI：
10.1016/j.jchromb.2018.04.029
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发表时间：
2018-06-15
期刊：
影响因子：
--
通讯作者：
Parthasarathy S
中科院分区：
文献类型：
--
作者：
Deme P;Narasimhulu CA;Parthasarathy S
我们以前报道，芝麻油（SO）在体内具有抗炎，抗动脉粥样硬化和降脂特性。我们最近的研究表明，芝麻油的水提取物（SOAE）也具有抗炎和抗动脉粥样硬化的特性，但没有降脂作用。动脉粥样硬化的减少程度使我们能够识别SOAE的成分并评估其体外抗炎特性。采用液相色谱-质谱联用技术对SOAE的组分进行了检测和鉴定。检测到甲氧基苯酚衍生物、短链和长链羧酸、二羧酸、羟基和氧代羧酸。令我们惊讶的是，芝麻素及其衍生物（木脂素），不存在于SOAE。在所鉴定的化合物中，选择甲氧基苯酚化合物的组合并测试它们降低LPS诱导的炎性基因表达的能力。将单核细胞衍生的巨噬细胞/RAW 264.7巨噬细胞用这些化合物预处理2小时，然后LPS刺激24小时，并分析促炎基因表达。这些甲氧基苯酚衍生物显示出有效的抗炎特性。总之，与SO相关的抗炎分子可能有助于抗炎和抗动脉粥样硬化特性。此外，我们的研究结果为开发基于SOAE的非药物治疗，营养补充剂和健康产品的各种炎症性疾病的未来。
We previously reported that sesame oil (SO) has anti-inflammatory, anti-atherosclerotic and lipid lowering properties in vivo. Our recent studies have shown that, an aqueous extract of sesame oil (SOAE) has also anti-inflammatory and anti-atherosclerotic properties but with no lipid lowering effects. The extent of reduction in atherosclerosis led us to identify components of SOAE and evaluate their anti-inflammatory properties in vitro. Liquid chromatography mass spectrometric method was used to detect and identify components of SOAE. Methoxyphenol derivatives, short and long chain carboxylic acids, dicarboxylic acids, hydroxy and oxo-carboxylic acids were detected. To our surprise, sesamol and its derivatives (lignans), were not present in the SOAE. Among the identified, a combination of methoxy phenol compounds were seleted and tested their ability to reduce LPS induced inflammatory gene expression. Monocyte derived macrophages/RAW 264.7 macrophages were pre-treated with these compounds for 2 hrs, followed by LPS stimulation for 24 hrs and pro-inflammatory gene expressions were analyzed. These methoxyphenol derivatives showed potent anti-inflammatory properties. In conclusion, the anti-inflammatory molecules associated with SO may contribute the anti-inflammatory and anti-atherosclerotic properties. Also, our results shed light for the development of SOAE based non-pharmacological therapeutics, nutritional supplements and health products for various inflammatory diseases in the future.
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