Detection of cancerous cervical cells using physical adhesion of fluorescent silica particles and centripetal force.
Detection of cancerous cervical cells using physical adhesion of fluorescent silica particles and centripetal force.
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DOI：
10.1039/c0an00366b
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发表时间：
2011-04-07
期刊：
影响因子：
--
通讯作者：
Sokolov I
中科院分区：
文献类型：
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作者：
Gaikwad RM;Dokukin ME;Iyer KS;Woodworth CD;Volkov DO;Sokolov I
在这里，我们描述了一种非传统的方法，以确定癌的人宫颈上皮细胞在培养皿中的基础上，二氧化硅珠和细胞之间的物理相互作用。它是一种简单的基于光学荧光的技术，其检测物理粘附到癌细胞和正常宫颈细胞表面的荧光二氧化硅珠的量的相对差异。该方法利用旋转培养皿中出现的向心力梯度。由于粘附力和向心力之间的平衡的变化，癌细胞和正常细胞在培养皿上显示出粘附于细胞表面的荧光颗粒的明显不同的分布。该方法证明与癌细胞相比，二氧化硅颗粒对正常细胞的粘附性更高。最初通过原子力显微镜（AFM）观察到粘附力的差异。AFM数据被用来设计旋转盘实验的参数。我们描述的光学方法比AFM更快，技术上更简单。这项工作提供了物理相互作用可以用来准确区分正常细胞和癌细胞的概念的证据。
Here we describe a non-traditional method to identify cancerous human cervical epithelial cells in a culture dish based on physical interaction between silica beads and cells. It is a simple optical fluorescence-based technique which detects the relative difference in the amount of fluorescent silica beads physically adherent to surfaces of cancerous and normal cervical cells. The method utilizes the centripetal force gradient that occurs in a rotating culture dish. Due to the variation in the balance between adhesion and centripetal forces, cancerous and normal cells demonstrate clearly distinctive distributions of the fluorescent particles adherent to the cell surface over the culture dish. The method demonstrates higher adhesion of silica particles to normal cells compared to cancerous cells. The difference in adhesion was initially observed by atomic force microscopy (AFM). The AFM data were used to design the parameters of the rotational dish experiment. The optical method that we describe is much faster and technically simpler than AFM. This work provides proof of the concept that physical interactions can be used to accurately discriminate normal and cancer cells.
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影响因子：
1.3
作者：
Hoda, RS;Colello, C;Houser, PM
通讯作者：
Houser, PM
DOI：
10.1007/s00249-004-0412-5
发表时间：
2004-11-01
影响因子：
2
作者：
Lekka, M;Laidler, P;Pyka, G
通讯作者：
Pyka, G
影响因子：
--
作者：
Costa KD
通讯作者：
Costa KD
影响因子：
--
作者：
Sokolov, I.;Volkov, D. O.
通讯作者：
Volkov, D. O.
影响因子：
38.3
作者：
通讯作者：
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