The host-parasite interface in molluscan schistosomiasis: biotin as a probe for sporocyst and hemocyte surface peptides.
The host-parasite interface in molluscan schistosomiasis: biotin as a probe for sporocyst and hemocyte surface peptides.
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软体动物血吸虫病中的宿主-寄生虫界面：生物素作为孢子被和血细胞表面肽的探针。
DOI：
10.1016/0304-4017(88)90121-5
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发表时间：
1988
影响因子：
2.6
通讯作者：
Hull,CJ
中科院分区：
文献类型：
--
作者：
Bayne,CJ;Hull,CJ
Hemocytes ofBiomphalaria glabratamediate the internal defensive response which, in resistant snail strains, kills sporocysts ofSchistosoma mansoni. Lacking a gut, the sporocyst has only its tegument to interact with the host milieu (hemolymph). We have, therefore, focused our study on the surface-exposed proteins of hemocytes and sporocyst tegument. Using gentle biotinylation of living systems, labelled proteins were studied after SDS-PAGE electrophoresis and transfer to nitrocellulose. Results validate the utility of surface biotinylation in studies of host and parasite interfaces. A low diversity characterizes hemocyte surfaces and strain-specific deferences are not in evidence. Hemocyte surfaces differ distinctly from the plasma in which these cells reside. In contrast, sporocyst surfaces expose a wide variety of peptides. These are remarkably stable even when sporocysts procured in snail plasma-free media are exposed to plasma. Thus, antigenic differences seen previously when Western immunoblotting was used to study sporocyst surfaces appear to be manifestations of minor changes in the exposed peptides or changes not detectable with this methodology. Hemoglobin, acquired by sporocysts from snail plasma, is processed and disappears from the surface during an overnight chase in culture medium.
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Biomphalaria glabrata（腹足纲）血细胞的表面抗原：一些血淋巴样表面抗原和带有 Con A 受体的大分子的连锁独立性的证据。
影响因子：
3.4
作者：
Yoshino,TP;Davis,CD
通讯作者：
Davis,CD
光滑双脐动物（腹足纲）血细胞的表面抗原：与蜗牛血红蛋白抗原相关的膜相关血淋巴样因子的出现。
影响因子：
3.4
作者：
Yoshino,TP
通讯作者：
Yoshino,TP
曼氏血吸虫原代孢子被皮表面蛋白分析。
DOI：
--
发表时间：
1987
期刊：
The Journal of parasitology
影响因子：
--
作者：
Boswell,CA;Yoshino,TP;Dunn,TS
通讯作者：
Dunn,TS
红细胞膜 3：最新临床和实验进展
DOI：
--
发表时间：
1985
期刊：
影响因子：
--
作者：
G. Hudson
通讯作者：
G. Hudson
影响因子：
2.2
作者：
BAYNE, CJ;LOKER, ES;STEPHENS, JA
通讯作者：
STEPHENS, JA