Culture-independent investigation of the microbiome associated with the nematode Acrobeloides maximus.
Culture-independent investigation of the microbiome associated with the nematode Acrobeloides maximus.
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DOI：
10.1371/journal.pone.0067425
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Orwin PM
中科院分区：
文献类型：
--
作者：
Baquiran JP;Thater B;Sedky S;De Ley P;Crowley D;Orwin PM
后生动物和微生物之间的共生关系广泛存在，对许多生态系统至关重要。最近对几种线虫的研究表明，与微生物共生体的密切联系也可能在这一门的成员中很常见。在这项工作中，我们探讨了可能的共生细菌和自由生活的土壤食菌线虫Acrobeloides maximus。我们采用土壤微宇宙的方法来暴露A。在RFP标记的大肠杆菌的土壤泥浆中生长的大菱鲆种群单菌。通过密度梯度分离回收蠕虫，并使用非培养和分离方法进行检查。一个16S rRNA基因调查的蠕虫相关的细菌进行了比较，土壤和一个类似的分析，使用秀丽隐杆线虫N2。无菌A.在胆固醇琼脂上保持大菱鲆种群，并取样以检查微生物组的种群动态。从A. maximus的形态特征与土壤或C. elegans相关集仅在A中发现了高水平的三个属：苍白杆菌属、土壤杆菌属和噬几丁杆菌属。maximus种群的多样性低于C.优雅假定的共生体群体在接种后至少维持4个月，尽管水平随着培养物老化而降低。荧光原位杂交（FISH）使用特异性探针对甲醛固定的线虫肠道中的细菌细胞进行染色。从土壤微宇宙中回收时，反复观察到三种微生物与Acrobeloides maximus。我们分离出几种苍白杆菌属和土壤杆菌属，并通过FISH证明它们栖息在线虫的肠道中。虽然他们在A. maximus的问题还没有解决，我们提出了这些细菌在保护宿主免受病原体侵害和促进其他摄入细菌的酶消化方面可能的互利作用。
Symbioses between metazoans and microbes are widespread and vital to many ecosystems. Recent work with several nematode species has suggested that strong associations with microbial symbionts may also be common among members of this phylu. In this work we explore possible symbiosis between bacteria and the free living soil bacteriovorous nematode Acrobeloides maximus. We used a soil microcosm approach to expose A. maximus populations grown monoxenically on RFP labeled Escherichia coli in a soil slurry. Worms were recovered by density gradient separation and examined using both culture-independent and isolation methods. A 16S rRNA gene survey of the worm-associated bacteria was compared to the soil and to a similar analysis using Caenorhabditis elegans N2. Recovered A. maximus populations were maintained on cholesterol agar and sampled to examine the population dynamics of the microbiome. A consistent core microbiome was extracted from A. maximus that differed from those in the bulk soil or the C. elegans associated set. Three genera, Ochrobactrum, Pedobacter, and Chitinophaga, were identified at high levels only in the A. maximus populations, which were less diverse than the assemblage associated with C. elegans. Putative symbiont populations were maintained for at least 4 months post inoculation, although the levels decreased as the culture aged. Fluorescence in situ hybridization (FISH) using probes specific for Ochrobactrum and Pedobacter stained bacterial cells in formaldehyde fixed nematode guts. Three microorganisms were repeatedly observed in association with Acrobeloides maximus when recovered from soil microcosms. We isolated several Ochrobactrum sp. and Pedobacter sp., and demonstrated that they inhabit the nematode gut by FISH. Although their role in A. maximus is not resolved, we propose possible mutualistic roles for these bacteria in protection of the host against pathogens and facilitating enzymatic digestion of other ingested bacteria.
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影响因子：
4.4
作者：
An R;Sreevatsan S;Grewal PS
通讯作者：
Grewal PS
影响因子：
14.9
作者：
Loy A;Maixner F;Wagner M;Horn M
通讯作者：
Horn M
DOI：
10.1099/00221287-146-3-709
发表时间：
2000-03-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Babic, I;Fischer-Le Saux, M;Boemare, N
通讯作者：
Boemare, N
DOI：
10.1073/pnas.0800072105
发表时间：
2008-03-11
影响因子：
11.1
作者：
Demaneche, Sandrine;Sanguin, Herve;Simonet, Pascal
通讯作者：
Simonet, Pascal
影响因子：
5.1
作者：
Friedrich, U;Van Langenhove, H;Lipski, A
通讯作者：
Lipski, A