Antibacterial activity of Artemisia nilagirica leaf extracts against clinical and phytopathogenic bacteria.
Antibacterial activity of Artemisia nilagirica leaf extracts against clinical and phytopathogenic bacteria.
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DOI：
10.1186/1472-6882-10-6
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发表时间：
2010-01-29
影响因子：
--
通讯作者：
Hopper W
中科院分区：
文献类型：
--
作者：
Ahameethunisa AR;Hopper W
筛选出6种青蒿素有机溶剂提取物对植物病原菌和临床重要标准参照菌的潜在抗菌活性。采用琼脂纸片扩散法研究了尼拉吉曲霉提取物对15株细菌的抑菌活性。用琼脂二倍稀释法测定其最低抑菌浓度(32~512μg/ml)，并对其主要植物化学衍生物进行植物化学筛选。除肺炎克雷伯菌、粪肠球菌和金黄色葡萄球菌外，其余提取物对革兰氏阳性菌和革兰氏阴性菌均有抑制作用。正己烷提取物对所有植物病原菌均有抑制作用，其最低抑菌浓度为32μg/ml；甲醇提取物对大肠埃希菌、小肠结肠炎耶尔森菌、伤寒沙门氏菌、产气性肠杆菌、普通变形杆菌、铜绿假单胞菌(32μg/ml)、枯草杆菌(μg/ml)和亚麻志贺菌(12 8μg/ml)有较高的抑菌活性。提取物的植物化学筛选为生物碱、氨基酸、黄酮类、苯酚、奎宁、单宁和萜类的主要衍生物。所有提取物对供试菌株均有抑菌活性。其中，甲醇提取物和正己烷提取物分别对临床病原菌和植物病原菌有较强的抑制作用。结果还表明，尼拉吉香草提取物中存在主要的植物化学衍生物。因此，从尼拉吉香草中分离和纯化具有治疗作用的潜在化合物可作为治疗人类和植物细菌性疾病的有效来源。
The six organic solvent extracts of Artemisia nilagirica were screened for the potential antimicrobial activity against phytopathogens and clinically important standard reference bacterial strains. The agar disk diffusion method was used to study the antibacterial activity of A. nilagirica extracts against 15 bacterial strains. The Minimum Inhibitory Concentration (MIC) of the plant extracts were tested using two fold agar dilution method at concentrations ranging from 32 to 512 μg/ml. The phytochemical screening of extracts was carried out for major phytochemical derivatives in A. nilagirica. All the extracts showed inhibitory activity for gram-positive and gram-negative bacteria except for Klebsiella pneumoniae, Enterococcus faecalis and Staphylococcus aureus. The hexane extract was found to be effective against all phytopathogens with low MIC of 32 μg/ml and the methanol extract exhibited a higher inhibition activity against Escherichia coli, Yersinia enterocolitica, Salmonella typhi, Enterobacter aerogenes, Proteus vulgaris, Pseudomonas aeruginosa (32 μg/ml), Bacillus subtilis (64 μg/ml) and Shigella flaxneri (128 μg/ml). The phytochemical screening of extracts answered for the major derivative of alkaloids, amino acids, flavonoids, phenol, quinines, tannins and terpenoids. All the extracts showed antibacterial activity against the tested strains. Of all, methanol and hexane extracts showed high inhibition against clinical and phytopathogens, respectively. The results also indicate the presence of major phytochemical derivatives in the A. nilagirica extracts. Hence, the isolation and purification of therapeutic potential compounds from A. nilagirica could be used as an effective source against bacterial diseases in human and plants.
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影响因子：
5.4
作者：
Mueller, MS;Karhagomba, IB;Wemakor, E
通讯作者：
Wemakor, E
影响因子：
5.4
作者：
Fernandez, MA;Garcia, MD;Saenz, MT
通讯作者：
Saenz, MT
影响因子：
5.4
作者：
Chakraborty, A;Brantner, AH
通讯作者：
Brantner, AH
DOI：
10.3923/pjbs.2008.1286.1289
发表时间：
2008-05-01
期刊：
Pakistan journal of biological sciences : PJBS
影响因子：
--
作者：
Maleki, S;Seyyednejad, S M;Motamedi, H
通讯作者：
Motamedi, H
影响因子：
1.5
作者：
Akhtar, MA;RahberBhatti, MH;Aslam, M
通讯作者：
Aslam, M