Noninvasive Assessment of Carotid Plaques Calcification by 18F-Sodium Fluoride Accumulation: Correlation with Pathology.
Noninvasive Assessment of Carotid Plaques Calcification by 18F-Sodium Fluoride Accumulation: Correlation with Pathology.
复制标题
通过 18F-氟化钠积累对颈动脉斑块钙化进行无创评估：与病理学的相关性。
DOI：
10.1016/j.jstrokecerebrovasdis.2018.02.011
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Juan Li
中科院分区：
文献类型：
--
作者：
Yan Zhang;Hong Li;Yingqin Jia;Pengfei Yang;Feng Zhao;Wei Wang;Wenqing Liu;Guisheng Chen;Xiaoqing Zhuang;Juan Li
背景血管钙化是目前公认的动脉粥样硬化的重要病理生物学过程，但其机制尚不清楚。鉴于血管钙化和骨形成的相似性，18F-氟化钠(18F-NaF)现在被认为是一种新的血管钙化标志物。本研究旨在探讨颈动脉斑块中18F-NaF积聚与血管钙化的组织学特征之间的关系。方法8例颈动脉狭窄患者接受颈动脉内膜切除术。CEA前进行18F-NaF正电子发射断层扫描和计算机断层扫描(PET-CT)，通过最大标准摄取值和靶-本底比测量18F-NaF摄取。同时测量了Hounsfield单位(HU)值。术后用苏木精-伊红染色、茜素红染色和免疫组织化学方法(α-平滑肌肌动蛋白和CD68)观察颈动脉斑块。与病理结果比较，颈动脉斑块的示踪剂活性与相应组织切片的钙化程度显著相关(积分光密度：r = .781，P= .022；阳性面积：r = .765，P= .027)。18F-NaF摄取与平滑肌细胞染色呈负相关(IOD：R = −.710，P= .049)。18F-NaF摄取与颈动脉狭窄、HU值及炎症无关。结论18F-NaF PET-CT是一种无创性评价颈动脉粥样硬化斑块钙化的成像方法，是研究动脉粥样硬化病变钙化的一种有前景的方法。
BackgroudVascular calcification is currently recognized as an important pathobiological process in atherosclerosis, but the mechanism remains elusive. Given the similarities in vascular calcification and bone formation,18F-sodium fluoride (18F-NaF) is now considered a novel marker of vascular calcification. This study aimed to correlate18F-NaF accumulation with the histological characterization of vascular calcification in carotid plaques.MethodsA total of 8 patients who were undergoing carotid endarterectomy (CEA) for carotid artery stenosis were recruited. Before CEA,18F-NaF positron emission tomography and computed tomography (PET-CT) studies were conducted.18F-NaF uptake was measured by the maximum standardized uptake value and the target-to-background ratio. The Hounsfield unit (HU) value was also measured. Postoperative carotid plaques were investigated by hematoxylin and eosin staining, alizarin red staining, and immunohistochemistry (alpha-smooth muscle actin and CD68).Results18F-NaF uptake was observed in the bilateral carotid bifurcation of all patients. Compared with the pathology results, there was a significant correlation between tracer activity in the carotid plaques and the calcification in the corresponding histological sections (integrated optical density [IOD]: r = .781,P= .022; positive area: r = .765,P= .027). A negative correlation was observed between18F-NaF uptake and smooth muscle cell staining (IOD: r = −.710,P= .049).18F-NaF uptake did not correlate with carotid artery stenosis, HU value, or inflammation.Conclusions18F-NaF PET-CT is a noninvasive imaging method for the assessment of calcification in human carotid atherosclerotic plaques and a promising approach to studying calcification in atherosclerotic lesions.
影响因子：
9.3
作者：
Leopold JA
通讯作者：
Leopold JA
影响因子：
20.1
作者：
Towler DA;Demer LL
通讯作者：
Demer LL