Complete chloroplast genome studies of different apple varieties indicated the origin of modern cultivated apples from Malus sieversii and Malus sylvestris.
Complete chloroplast genome studies of different apple varieties indicated the origin of modern cultivated apples from Malus sieversii and Malus sylvestris.
复制标题
不同苹果品种的完整叶绿体基因组研究表明现代栽培苹果的起源来自Malus sieversii和Malus sylvestris
作者：
Li X;Ding Z;Miao H;Bao J;Tian X
背景苹果是世界上最重要的温带落叶果树之一，栽培范围广泛。在这项研究中，我们评估了野生和栽培苹果（苹果属）的完整叶绿体基因组之间的变异和系统发育关系。方法利用新一代测序技术获得了24个苹果品种的叶绿体全基因组，并与野生种（从NCBI下载）的基因组进行比较。结果苹果属植物叶绿体基因组高度保守，基因组长度为160，067 ~ 160，290 bp，均为双链环状四分体结构。野生苹果和栽培苹果基因组的基因含量和序列基本相同，但在基因组中检测到多个突变热点区域（psbI-atpA、psbM-psbD和ndhC-atpE）。这些区域可为分类学研究中解决特异性分子标记提供有价值的信息。系统发育分析表明，苹果属形成了一个新的分支，4个栽培品种与苹果属聚为一个分支。sylvestris和M. sieversii，表明M. sylvestris和M. sieversii是栽培苹果的祖先种。
Background Apple is one of the most important temperate deciduous fruit trees worldwide, with a wide range of cultivation. In this study, we assessed the variations and phylogenetic relationships between the complete chloroplast genomes of wild and cultivated apples (Malus spp.). Method We obtained the complete chloroplast genomes of 24 apple varieties using next-generation sequencing technology and compared them with genomes of (downloaded from NCBI) the wild species. Result The chloroplast genome of Malus is highly conserved, with a genome length of 160,067–160,290 bp, and all have a double-stranded circular tetrad structure. The gene content and sequences of genomes of wild species and cultivated apple were almost the same, but several mutation hotspot regions (psbI-atpA, psbM-psbD, and ndhC-atpE) were detected in these genomes. These regions can provide valuable information for solving specific molecular markers in taxonomic research. Phylogenetic analysis revealed that Malus formed a new clade and four cultivated varieties clustered into a branch with M. sylvestris and M. sieversii, which indicated that M. sylvestris and M. sieversii were the ancestor species of the cultivated apple.
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DOI：
10.3390/molecules23092137
发表时间：
2018-08-24
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Meng XX;Xian YF;Xiang L;Zhang D;Shi YH;Wu ML;Dong GQ;Ip SP;Lin ZX;Wu L;Sun W
通讯作者：
Sun W
影响因子：
4.5
作者：
Cornille A;Gladieux P;Smulders MJ;Roldán-Ruiz I;Laurens F;Le Cam B;Nersesyan A;Clavel J;Olonova M;Feugey L;Gabrielyan I;Zhang XG;Tenaillon MI;Giraud T
通讯作者：
Giraud T
影响因子：
2.5
作者：
Lohse, Marc;Drechsel, Oliver;Bock, Ralph
通讯作者：
Bock, Ralph
影响因子：
64.8
作者：
OHYAMA, K;FUKUZAWA, H;OZEKI, H
通讯作者：
OZEKI, H
影响因子：
3.7
作者：
Dong W;Xu C;Cheng T;Zhou S
通讯作者：
Zhou S