Stem cells and tissue-engineered skin.

Stem cells and tissue-engineered skin.
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DOI:
10.1159/000178864
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发表时间:
2009
影响因子:
2.7
通讯作者:
Ghadially R
Ghadially R
中科院分区:
医学4区
文献类型:
--
作者:
Charruyer A;Ghadially R

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皮肤和全身疾病的临床应用(如创伤修复和基因治疗)需要皮肤组织工程的进展。本文综述了利用表皮干细胞作为细胞来源改善组织工程皮肤的研究进展。我们讨论了使用生物标记物分离表皮干细胞的重要性和局限性,以寻求一种纯粹的干细胞制备,以及保持这种纯度所需的培养条件,以获得质量更好和持久的工程皮肤。最后,我们回顾了使用额外的多潜能干细胞来源在功能和美观上优化工程化组织的优势。
Advances in tissue engineering of skin are needed for clinical applications (as in wound healing and gene therapy) for cutaneous and systemic diseases. In this paper we review the use of epidermal stem cells as a source of cells to improve tissue-engineered skin. We discuss the importance and limitations of epidermal stem cell isolation using biomarkers, in quest of a pure stem cell preparation, as well as the culture conditions necessary to maintain this purity as required for a qualitatively superior and long-lasting engineered skin. Finally, we review the advantages of using additional multipotent stem cell sources to functionally and cosmetically optimize the engineered tissue.
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