The Lentiviral System Construction for Highly Expressed Porcine Stearoyl-CoA Desaturase-1 and Functional Characterization in Stably Transduced Porcine Swine Kidney Cells.
The Lentiviral System Construction for Highly Expressed Porcine Stearoyl-CoA Desaturase-1 and Functional Characterization in Stably Transduced Porcine Swine Kidney Cells.
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DOI：
10.1002/lipd.12102
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发表时间：
2018-10
期刊：
影响因子：
1.9
通讯作者：
Smith, Stephen B.
中科院分区：
文献类型：
--
作者：
Hwang, Jinhee;Singh, Neetu;Long, Charles;Smith, Stephen B.
动物组织中最受调控和最丰富的脂肪酸是油酸(18：1n9)。油酸是由Δ9去饱和酶、硬脂酰辅酶A去饱和酶-1合成的，它负责合成可能的细胞因子棕榈油酸(16：1N7)和18：2顺-9，反-11共轭亚油酸。鉴于SCD1在脂类代谢中的重要作用，我们利用慢病毒表达系统建立了猪肾(SK6)转基因细胞系，用于猪硬脂酰辅酶A脱饱和酶-1(PSCD1)的持续高表达或可诱导表达。我们成功地验证了这些细胞培养模型中pSCD1的表达和功能，证明了PSCD转导的细胞过度表达pSCD1蛋白和mRNA。此外，转导pSCD1的细胞将棕榈酸(16：0)转化为棕榈油酸的能力提高了近四倍。本研究中使用的慢病毒载体可进一步用于产生转基因动物，以证明SCD1过表达对肥胖和脂肪变性的影响。
The most highly regulated and abundant fatty acid in animal tissue is oleic acid (18:1n9). Oleic acid is synthesized by the Δ9 desaturase, stearoyl-CoA desaturase-1 (SCD1), which is responsible for the synthesis of the putative cytokine palmitoleic acid (16:1n7) and 18:2 cis-9, trans-11 conjugated linoleic acid. Owing to the importance of SCD1 in lipid metabolism, we generated porcine swine kidney (SK6) transgenic cell lines for sustained overexpression or knockdown of porcine stearoyl-CoA desaturase-1 (pSCD1) in an inducible manner by utilizing a lentiviral expression system. We successfully validated these cell culture models for expression and functionality of pSCD1 by documenting that the pSCD-transduced cells overexpressed pSCD1 protein and mRNA. Additionally, the pSCD1-transduced cells increased the conversion of palmitate (16:0) to palmitoleic acid nearly fourfold. The lentiviral vectors utilized in this study can be further used to generate transgenic animals to document the effects of the overexpression of SCD1 on obesity and steatosis.
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影响因子：
4.5
作者：
Gilmore, L. Anne;Crouse, Stephen F.;Smith, Stephen B.
通讯作者：
Smith, Stephen B.
影响因子：
4.2
作者：
Gilmore, L. Anne;Walzem, Rosemary L.;Smith, Stephen B.
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影响因子：
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作者：
Koponen, JK;Kankkonen, H;Ylä-Herttuala, S
通讯作者：
Ylä-Herttuala, S
DOI：
10.1073/pnas.96.6.2988
发表时间：
1999-03-16
影响因子：
11.1
作者：
Case, SS;Price, MA;Crooks, GM
通讯作者：
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影响因子：
3.3
作者：
Chung, K. Y.;Lunt, D. K.;Smith, S. B.
通讯作者：
Smith, S. B.