Enhanced mechanical performance and biological evaluation of a PLGA coated β-TCP composite scaffold for load-bearing applications.
Enhanced mechanical performance and biological evaluation of a PLGA coated β-TCP composite scaffold for load-bearing applications.
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DOI：
10.1016/j.eurpolymj.2011.05.004
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发表时间：
2011-08-01
影响因子：
6
通讯作者：
Yang, Yunzhi
中科院分区：
文献类型：
--
作者：
Kang, Yunqing;Scully, Allison;Young, Daniel A.;Kim, Sungwoo;Tsao, Helen;Sen, Milan;Yang, Yunzhi
关键词：
多孔β-磷酸三钙（β-TCP）具有良好的生物相容性、骨传导性和生物可降解性，已被临床用于骨修复和骨置换。然而，β-TCP的脆性限制了其应用。在这里，我们证明了与未涂覆的β-TCP支架相比，渗透有聚（乳酸-羟基乙酸共聚物）（PLGA）聚合物薄层的互连多孔β-TCP支架显示出改善的机械性能，同时保持其优异的互连性和生物相容性。PLGA的渗透使β-TCP支架的压缩强度从2.90 MPa增加到4.19 MPa，弯曲强度从1.46 MPa增加到2.41 MPa，韧性从0.17 MPa增加到1.44 MPa，同时保持了互连的多孔结构，孔隙率为80.65%。PLGA涂层β-TCP支架的机械性能的这些显著改善是由于支柱的系统涂层、互穿结构特征和裂纹桥接的组合。体外生物学评价结果表明，大鼠骨髓基质细胞（rBMSCs）在PLGA包被的β-TCP和β-TCP上均能良好粘附、增殖，并表达碱性磷酸酶（ALP）活性。这些结果提示了一种新的策略，用于制造具有显著增强的机械强度的互连大孔支架，用于潜在的承重骨组织再生。
Porous β-tricalcium phosphate (β-TCP) has been used for bone repair and replacement in clinics due to its excellent biocompatibility, osteoconductivity, and biodegradability. However, the application of β-TCP has been limited by its brittleness. Here, we demonstrated that an interconnected porous β-TCP scaffold infiltrated with a thin layer of poly (lactic-co-glycolic acid) (PLGA) polymer showed improved mechanical performance compared to an uncoated β-TCP scaffold while retaining its excellent interconnectivity and biocompatibility. The infiltration of PLGA significantly increased the compressive strength of β-TCP scaffolds from 2.90 MPa to 4.19 MPa, bending strength from 1.46 MPa to 2.41 MPa, and toughness from 0.17 MPa to 1.44 MPa, while retaining an interconnected porous structure with a porosity of 80.65%. These remarkable improvements in the mechanical properties of PLGA-coated β-TCP scaffolds are due to the combination of the systematic coating of struts, interpenetrating structural characteristics, and crack bridging. The in vitro biological evaluation demonstrated that rat bone marrow stromal cells (rBMSCs) adhered well, proliferated, and expressed alkaline phosphatase (ALP) activity on both the PLGA-coated β-TCP and the β-TCP. These results suggest a new strategy for fabricating interconnected macroporous scaffolds with significantly enhanced mechanical strength for potential load-bearing bone tissue regeneration.
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影响因子：
14
作者：
Kim, HW;Knowles, JC;Kim, HE
通讯作者：
Kim, HE
影响因子：
2.4
作者：
GIBSON, LJ
通讯作者：
GIBSON, LJ
影响因子：
14
作者：
Kim, SS;Park, MS;Kim, BS
通讯作者：
Kim, BS
影响因子：
2.2
作者：
Rho, JY;Kuhn-Spearing, L;Zioupos, P
通讯作者：
Zioupos, P
DOI：
10.1023/b:jmsm.0000021113.88702.9d
发表时间：
2004-04-01
影响因子：
3.7
作者：
Navarro, M;Ginebra, MP;Ambrosio, L
通讯作者：
Ambrosio, L