Screening and characterization of sex-linked DNA markers and marker-assisted selection in blue tilapia (Oreochromis aureus)
Screening and characterization of sex-linked DNA markers and marker-assisted selection in blue tilapia (Oreochromis aureus)
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蓝色罗非鱼 (Oreochromis aureus) 性连锁 DNA 标记的筛选和表征以及标记辅助选择
DOI：
10.1016/j.aquaculture.2020.735934
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发表时间：
2021-01
期刊：
影响因子：
4.5
通讯作者：
Wang D. S.
中科院分区：
文献类型：
--
作者：
Wu X;Zhao L;Fan Z;Lu B. Y;Chen J. L;Tan D. J;Jiang D. N;Tao W. J;Wang D. S.
奥利亚罗非鱼（Oreochromis aureus）是世界上重要的养殖鱼类，其雌雄个体大小差异较大，雌雄个体的个体差异较大，因此进行单性别养殖具有重要的应用价值。然而，由于缺乏准确的蓝罗非鱼性别连锁标记，阻碍了蓝罗非鱼单性别养殖的发展，也阻碍了蓝罗非鱼性别决定的研究。本研究对WW超雌系进行了选育。通过对5个品系的ZZ/ZW基因组重测序数据与ZZ/WW参考基因组序列的比较分析，确定了蓝罗非鱼性别决定区WW和ZZ之间的结构差异。根据这些结构变异，设计了30对引物筛选性连锁标记。经过两轮PCR筛选，获得3个可用于蓝罗非鱼遗传性别鉴定的标记，分别命名为Marker-1、Marker-2和Marker-3。将Marker-2和Marker-3组合在一起进行多重PCR扩增，以区分ZZ、ZW和WW鱼，弥补共显性标记的不足。综上所述，本研究筛选出3个与性别连锁的标记，用于蓝罗非鱼不同基因型的鉴定和MAS-GMT（marker-assisted selection of genetically male tilapias）技术的建立。这对于分离性别决定基因、阐明性染色体的进化和性别决定系统的过渡具有重要意义。
The blue tilapia (Oreochromis aureus) is an important cultured fish worldwide, and exhibits sexual size dimorphism in favor of males, and therefore, mono-sex breeding is of great value for aquaculture. However, the lack of accurate sex-linked markers for blue tilapia has hampered the mono-sex culture in aquaculture, as well as study of sex determination in this species. In this study, WW super-females were bred and selected. Then, structure variations between WW and ZZ in sex determining region were identified from blue tilapia by comparative analysis of ZZ/ZW genome re-sequencing data from 5 different strains and ZZ/WW reference genome sequences. Based on these structure variations, 30 pairs of primers were designed to screen sex-linked markers. After two rounds of PCR screening, three markers, named as Marker-1, Marker-2 and Marker-3, were obtained to identify the genetic sex of blue tilapia. Marker-2 and Marker-3 were combined together for multiplex PCR amplification to distinguish ZZ, ZW and WW fish to make up for the lack of a co-dominant marker. In conclusion, three sex-linked markers were isolated and used for identification of different genotypes and establishment of MAS-GMT (marker-assisted selection of genetically male tilapias) technique in blue tilapia. It is also of great significance for isolation of sex determining gene, elucidation of the evolution of sex chromosomes and the transition of sex determination system.
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影响因子：
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作者：
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