Genetic and physical mapping of sex-linked AFLP markers in Nile tilapia (Oreochromis niloticus).

Genetic and physical mapping of sex-linked AFLP markers in Nile tilapia (Oreochromis niloticus).
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尼罗罗非鱼(Oreochromis niloticus)中性别连接的AFLP标记的遗传和物理图。

DOI:
10.1007/s10126-010-9326-7
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发表时间:
2011-06
影响因子:
3
通讯作者:
Kocher, Thomas D.
Kocher, Thomas D.
中科院分区:
生物学2区
文献类型:
--
作者:
Lee, Bo-Young;Coutanceau, Jean-Pierre;Ozouf-Costaz, Catherine;D'Cotta, Helena;Baroiller, Jean-Francois;Kocher, Thomas D.

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尼罗罗非鱼(Oreochromis niloticus)性别决定基因的鉴定对商业养殖具有重要意义。我们之前在该物种的连锁群1 (LG1)上的标记GM201和UNH995之间的10cM间隔内发现了一个XX/XY性别决定位点。为了完善这一区域,我们开发了新的AFLP标记使用散装分离分析的作图家族。我们确定了三个AFLP标记,显示出性别特异性的分离模式。这三种基因都位于先前确定的LG1上的性别决定区域附近,但就在其外围。将含有这些标记的BAC克隆与染色体扩增进行杂交,证实了O. niloticus的XX/XY性别决定位点位于一条小染色体上。
Identification of the sex-determining genes of the Nile tilapia (Oreochromis niloticus) has important implications for commercial aquaculture. We previously identified an XX/XY sex-determining locus in this species within a 10cM interval between markers GM201 and UNH995 on linkage group one (LG1). In order to refine this region, we developed new AFLP markers using bulked segregant analysis of the mapping families. We identified three AFLP markers that showed a sex-specific pattern of segregation. All three mapped near, but just outside, the previously identified sex-determining region on LG1. Hybridization of BAC clones containing these markers to chromosome spreads confirmed that the XX/XY sex determining locus is on one of the small chromosomes in O. niloticus.
DOI: 10.1159/000015484
发表时间: 2000-01-01
期刊: CYTOGENETICS AND CELL GENETICS
影响因子: --
作者:
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影响因子: 2.4
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