Different responses of transgenic Bt rice and conventional rice to elevated ozone concentration
Different responses of transgenic Bt rice and conventional rice to elevated ozone concentration
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转基因Bt水稻和常规水稻对臭氧浓度升高的不同反应
DOI：
10.1007/s11356-017-8508-5
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发表时间：
2017-02
影响因子：
5.8
通讯作者：
Liu Biao
中科院分区：
文献类型：
--
作者：
Li Chunhua;Zhu Jianguo;Zeng Qing;Luo Keju;Liu Biao
为探讨转Bt基因汕优63（Bt-SY 63）和非转Bt基因汕优63（SY 63）对臭氧（O3）敏感性的差异，采用盆栽试验，研究了环境O3浓度（A-O3）和高浓度O3（1.5 × A-O3，E-O3）对汕优63叶片气体交换、产量、籽粒元素和抗氧化酶活性的影响。A-O3胁迫下，Bt-SY 63的叶绿素含量和产量显著低于SY 63，而气孔导度（Gs）、胞内CO2浓度（Ci）和Fe、Zn浓度则相反。两品种间的丙二醛（MDA）含量差异不显著，但抗氧化酶活性（SOD、POD和CAT）在Bt SY 63中的活性有高于SY 63的趋势。E-O3胁迫导致两品种光合速率、产量和籽粒中除氮素外的其他元素含量显著下降，且Bt-SY 63的下降幅度远大于SY 63。同时，在E-O3下，Bt-SY 63和SY 63的MDA含量分别显著增加了30.6%和23.7%。这些结果表明，外源基因的插入可以诱导Bt-SY 63在生理和生长过程中发生一些无意的变化，与环境O3浓度下的SY 63相比。另外，Bt-SY 63的O3浓度升高对细胞的损伤程度比SY 63严重。本研究为Bt-SY 63在未来气候条件下的商业化释放和育种策略提供了有价值的基线信息。
To assess the different sensitivity to ozone (O3) between transgenic Bt Shanyou63 (Bt-SY63) and its nontransgenic counterpart Shanyou63 (SY63), the leaf gas exchange, yield, grain elements, and antioxidant enzymes were investigated by performing a pot experiment under ambient O3concentration (A-O3) and elevated O3concentration (1.5 × A-O3, E-O3). Under A-O3, the chlorophyll content and yield of Bt-SY63 were significantly lower than those of SY63, whereas the stomatal conductance (Gs), cellular CO2concentration (Ci), and Fe, Zn concentration showed the opposite trends. No significant difference was detected for malondialdehyde (MDA) content between two cultivars, although the antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities in Bt-SY63 tended to be higher than those in SY63. Exposure to E-O3resulted in significant reduction for photosynthesis (Pn), yield and all grain elements (except for N) concentration in both varieties, and the extents in Bt-SY63 were much greater than those in SY63. Meanwhile, significant increases for MDA content by 30.6 and 23.7% in Bt-SY63 and SY63, respectively, were detected under E-O3. These results demonstrated that insertion of exogenous gene could induce several unintentional changes of Bt-SY63 in physiology and growth progress, compared with SY63 under ambient O3concentration. On the other hand, the injury of Bt-SY63 caused by elevated O3concentration was more severe than that of SY63. This study provided valuable baseline information for the commercial release and breeding strategies of Bt-SY63 under the projected future climate.
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DOI：
10.1046/j.1469-8137.1998.00277.x
发表时间：
1998-10
期刊：
The New phytologist
影响因子：
--
作者：
C. Whitfield;A. Davison;T. Ashenden
通讯作者：
C. Whitfield;A. Davison;T. Ashenden
DOI：
--
发表时间：
2009
期刊：
Journal of Wuhan Botanical Research
影响因子：
--
作者：
Sun Jiawei;Zhao Tianhong;Fu Yu;C. Ying
通讯作者：
Sun Jiawei;Zhao Tianhong;Fu Yu;C. Ying
DOI：
10.3390/antiox2040340
发表时间：
2013-11-26
期刊：
Antioxidants (Basel, Switzerland)
影响因子：
--
作者：
Racchi ML
通讯作者：
Racchi ML
DOI：
10.1016/j.agee.2009.03.012
发表时间：
2009-08
期刊：
Agriculture, Ecosystems & Environment
影响因子：
--
作者：
J. Pang;K. Kobayashi;Jianguo Zhu
通讯作者：
J. Pang;K. Kobayashi;Jianguo Zhu
影响因子：
6.9
作者：
Mittova, V;Guy, M;Volokita, M
通讯作者：
Volokita, M