Three-dimensional fine structures of the maculae flavae of the human vocal fold using correlative light and electron microscopy.
Three-dimensional fine structures of the maculae flavae of the human vocal fold using correlative light and electron microscopy.
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DOI：
10.1002/lio2.950
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发表时间：
2022-12
影响因子：
1.9
通讯作者：
Umeno, Hirohito
中科院分区：
文献类型：
--
作者：
Sato, Kiminobu;Kurita, Takashi;Sato, Fumihiko;Ohta, Keisuke;Sato, Kiminori;Chitose, Shun-ichi;Ono, Takeharu;Umeno, Hirohito
关键词：
为了分析复杂组织的各个方面，在生物学中对不同长度尺度的每个样本进行研究的需求越来越大。相关光电子显微镜（CLEM）是将两种不同类型的信息关联到同一组织学领域的最新技术：组织学（来自光电子显微镜）和超微结构（来自电子显微镜）。本文用CLEM对人声襞黄斑的三维精细结构进行了研究。用化学消解法（改良氢氧化钠浸渍法）对5个正常成人声带标本进行石蜡包埋、切片和载玻片的研究。使用CLEM进行观察。光镜和场发射扫描电镜能够在相同的组织学区域观察到MFe及其周围区域的细胞和细胞外基质的精细结构。人声襞MFe中混杂有鹅卵石样多角形细胞、声带星状细胞样细胞和成纤维细胞样梭形细胞。在MFe中，三种类型细胞周围的细胞外基质不同，表明细胞的功能特性不同。CLEM是一种观察人声带粘膜三维精细结构的有效技术。本研究的结果与人类声带MFe细胞具有异质性的假设是一致的，三种类型的细胞具有不同的性质。利用相关光镜和电镜对人声带黄斑的三维精细结构进行了研究。
To analyze various aspects of complex tissue, there is increasing demand to study each sample at different length scales in biology. Correlative light and electron microscopy (CLEM) is the latest technique to correlate two different types of information on the exact same histological area of interest: histology (from light microscopy) and ultrastructure (from electron microscopy). The three‐dimensional fine structures of the maculae flavae (MFe) of the human vocal fold were investigated using CLEM. Five normal human adult vocal folds as specimens embedded in paraffin, sectioned, and mounted on glass slides with/without a chemical digestion method (modified sodium hydroxide maceration method) were investigated. Observations using CLEM were performed. The fine structures of cells and extracellular matrices in the MFe and their peripheral regions were able to be observed on the exact same histological area of interest with the light microscope and field emission‐scanning electron microscope. Cobblestone‐like polygonal cells, vocal fold stellate cell‐like cells, and fibroblast‐like spindle cells were intermingled in the MFe of the human vocal fold. The extracellular matrices surrounding each three types of cell in the MFe differed, suggesting the cells were different in functional property. CLEM is a useful technique to observe the three‐dimensional fine structures of the human vocal fold mucosa. The results of the present study are consistent with the hypothesis that the cells in the MFe of the human vocal fold have heterogeneity and each three types of cell have different properties. The three‐dimensional fine structures of the maculae flavae of the human vocal fold were investigated using correlative light and electron microscopy.
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影响因子：
--
作者：
Inaga, Sumire;Hirashima, Sayuri;Naguro, Tomonori
通讯作者：
Naguro, Tomonori
影响因子：
1.9
作者：
Sato K;Chitose SI;Sato F;Sato K;Ono T;Umeno H
通讯作者：
Umeno H
影响因子：
4.6
作者：
Sawaguchi A;Kamimura T;Yamashita A;Takahashi N;Ichikawa K;Aoyama F;Asada Y
通讯作者：
Asada Y
影响因子：
1.4
作者：
Sato, K;Hirano, M;Nakashima, T
通讯作者：
Nakashima, T
影响因子：
1.4
作者：
Sato, K;Hirano, M;Nakashima, T
通讯作者：
Nakashima, T