Heterogeneity and hierarchy of the tissue stem cells in the human newborn vocal fold mucosa.
Heterogeneity and hierarchy of the tissue stem cells in the human newborn vocal fold mucosa.
复制标题
DOI：
10.1002/lio2.470
复制
发表时间：
2020-10
影响因子：
1.9
通讯作者：
Umeno H
中科院分区：
文献类型：
--
作者：
Sato K;Chitose SI;Sato F;Sato K;Ono T;Umeno H
越来越多的证据表明，黄斑 (MFe) 中的细胞是组织干细胞，而 MFe 是人类声带粘膜的干细胞生态位。在体内研究了新生儿声带 MFe 中组织干细胞的异质性和层次结构。人类声带的组织学分析。在透射电子显微镜和光学显微镜下研究了五个正常人类新生儿声带。新生MFe体内的鹅卵石样多边形细胞、声带星状细胞样细胞和成纤维细胞样梭形细胞混合存在，表明MFe中的细胞具有异质性。然而，鹅卵石状的多边形细胞占主导地位。游离核糖体在细胞质中发育良好。在某些情况下，细胞彼此形成间隙连接。在某些情况下，细胞附着在其他细胞上并相互形成细胞连接。这些发现表明新生黄斑中的细胞具有间充质细胞（间充质细胞）的特征。观察到集落形成单位样细胞聚集体，表明新生 MFe 中的细胞具有干细胞性。鹅卵石状的多边形细胞表达 SSEA-3（一种人类多能干细胞标记物），表明它们位于干细胞系统细胞层次结构的顶部。人新生儿声带粘膜MFe中的细胞在体内干细胞系统中具有异质性和层次性。出生时，新生黄斑已准备好开始作为振动组织生长声带粘膜。
There is growing evidence that the cells in the maculae flavae (MFe) are tissue stem cells and the MFe are a stem cell niche of the human vocal fold mucosa. Heterogeneity and hierarchy of tissue stem cells in the MFe of newborn vocal fold were investigated in vivo. Histologic analysis of the human vocal folds. Five normal human newborn vocal folds were investigated under transmission electron microscopy and light microscopy. Cobblestone‐like polygonal cells, vocal fold stellate cell‐like cells, and fibroblast‐like spindle cells were intermingled in the newborn MFe in vivo, indicating that the cells in the MFe had heterogeneity. However, cobblestone‐like polygonal cells were predominant. Free ribosomes were well developed in the cytoplasm. The cells in some cases formed gap junctions with each other. The cells in some cases were attached to other cells and formed cell junctions with each other. These findings indicated cells in the newborn maculae flavae possessed features of mesenchymal cells (cells in mesenchyme). Colony‐forming‐unit‐like cell aggregate was observed, indicating the cells in the newborn MFe had stemness. The cobblestone‐like polygonal cells expressed SSEA‐3 (a human pluripotent stem cell marker), indicating they were at the top of a cellular hierarchy in the stem cell system. The cells in the MFe of the human newborn vocal fold mucosa had heterogeneity and hierarchy in the stem cell system in vivo. At birth, newborn maculae flavae are ready to start the growth of the vocal fold mucosa as a vibrating tissue.
登录
查看更多内容
DOI：
10.1007/978-3-319-69194-7_9
发表时间：
2017-01-01
期刊：
STEM CELL MICROENVIRONMENTS AND BEYOND
影响因子：
--
作者：
Sato, Kiminori
通讯作者：
Sato, Kiminori
影响因子：
2.6
作者：
Hanson, Summer E.;Kim, Jaehyup;Thibeault, Susan L.
通讯作者：
Thibeault, Susan L.
影响因子：
1.4
作者：
Yamashita, Masaru;Hirano, Shigeru;Ito, Juichi
通讯作者：
Ito, Juichi
影响因子：
1.4
作者：
Sato, K;Hirano, M;Nakashima, T
通讯作者：
Nakashima, T
喉粘膜间充质干细胞修复声带损伤的应用
影响因子：
14
作者：
Peng, Han;Ming, Leiguo;Deng, Zhihong
通讯作者：
Deng, Zhihong