Phospholipids that contain polyunsaturated fatty acids enhance neuronal cell mechanics and touch sensation.
Phospholipids that contain polyunsaturated fatty acids enhance neuronal cell mechanics and touch sensation.
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DOI：
10.1016/j.celrep.2013.12.012
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发表时间：
2014-01-16
期刊：
影响因子：
8.8
通讯作者：
Goodman MB
中科院分区：
文献类型：
--
作者：
Vásquez V;Krieg M;Lockhead D;Goodman MB
嵌入神经元细胞膜中的机械-电转导（MeT）通道对于触觉和本体感受是必不可少的。关于天然MeT通道和膜磷脂之间的相互作用知之甚少，部分原因是很少有技术可用于在体内改变质膜组成。在这里，我们利用遗传解剖，化学互补和光遗传学来建立花生四烯酸（AA），一种ω-6多不饱和脂肪酸，增强触觉和机电转导活性，同时纳入C膜磷脂。elegans触觉感受神经元（TRNs）。由于动态力光谱表明，AA调制TRN质膜的机械性能，我们建议，这种PUFA是需要的MeT通道活动。这些发现确立了多不饱和磷脂是机械感受神经元的生物化学和力学的关键决定因素，并加强了动物中感觉机械转导依赖于由蛋白质和膜脂质组成的细胞机器的想法。
Mechano-electrical transduction (MeT) channels embedded in neuronal cell membranes are essential for touch and proprioception. Little is understood about the interplay between native MeT channels and membrane phospholipids, in part because few techniques are available for altering plasma membrane composition in vivo. Here, we leverage genetic dissection, chemical complementation, and optogenetics to establish that arachidonic acid (AA), an omega-6 polyunsaturated fatty acid, enhances touch sensation and mechanoelectrical transduction activity while incorporated into membrane phospholipids in C. elegans touch receptor neurons (TRNs). Because dynamic force spectroscopy reveals that AA modulates the mechanical properties of TRN plasma membranes, we propose that this PUFA is needed for MeT channel activity. These findings establish that polyunsaturated phospholipids are crucial determinants of both the biochemistry and mechanics of mechanoreceptor neurons and reinforce the idea that sensory mechanotransduction in animals relies on a cellular machine composed of both proteins and membrane lipids.
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DOI：
10.1073/pnas.1302018110
发表时间：
2013-03-26
影响因子：
11.1
作者：
Anishkin, Andriy;Kung, Ching
通讯作者：
Kung, Ching
影响因子：
64.8
作者：
Chelur, DS;Ernstrom, GG;Chalfie, M
通讯作者：
Chalfie, M
影响因子：
2.7
作者：
CHALFIE, M;SULSTON, J
通讯作者：
SULSTON, J
影响因子：
2.9
作者：
Cortes, DM;Perozo, E
通讯作者：
Perozo, E
影响因子：
48
作者：
Calixto, Andrea;Chelur, Dattananda;Topalidou, Irini;Chen, Xiaoyin;Chalfie, Martin
通讯作者：
Chalfie, Martin