Whole-genome analysis of human influenza A virus reveals multiple persistent lineages and reassortment among recent H3N2 viruses.
Whole-genome analysis of human influenza A virus reveals multiple persistent lineages and reassortment among recent H3N2 viruses.
复制标题
DOI：
10.1371/journal.pbio.0030300
复制
发表时间：
2005-09
期刊：
影响因子：
9.8
通讯作者：
Taubenberger JK
中科院分区：
文献类型：
--
作者：
Holmes EC;Ghedin E;Miller N;Taylor J;Bao Y;St George K;Grenfell BT;Salzberg SL;Fraser CM;Lipman DJ;Taubenberger JK
了解人类甲型流感病毒的进化对于监测和疫苗株选择非常重要。我们对 1999 年至 2004 年间从美国纽约州收集的 156 个人类 H3N2 甲型流感病毒的完整基因组进行了系统发育分析，并观察到具有不同种群频率的多个共循环进化枝。引人注目的是，对单个基因片段推断的系统发育表明，这些分支之间发生了多次重配事件，因此至少自 2000 年以来存在的 H3N2 病毒的一个分支为 2002-2003 年流感季节后采样的所有 H3N2 病毒提供了血凝素基因。这种重配事件可能是导致 2003-2004 年流感季节 A/Fujian/411/2002 类流行病的抗原变异流感病毒株的祖先。然而，尽管共享相同的血凝素，这些系统发育上不同的病毒谱系仍继续在同一群体中共同传播。这些数据源自对 H3N2 病毒的首次大规模分析，令人信服地证明多个谱系可以以具有流行病学意义的方式共同循环、持续存在和重配，并强调了基因组分析对于未来流感监测的重要性。通过基因组系统发育分析流感病毒的进化；人们发现人类提供了隐含在流感适应和毒力中的抗原变异库。
Understanding the evolution of influenza A viruses in humans is important for surveillance and vaccine strain selection. We performed a phylogenetic analysis of 156 complete genomes of human H3N2 influenza A viruses collected between 1999 and 2004 from New York State, United States, and observed multiple co-circulating clades with different population frequencies. Strikingly, phylogenies inferred for individual gene segments revealed that multiple reassortment events had occurred among these clades, such that one clade of H3N2 viruses present at least since 2000 had provided the hemagglutinin gene for all those H3N2 viruses sampled after the 2002–2003 influenza season. This reassortment event was the likely progenitor of the antigenically variant influenza strains that caused the A/Fujian/411/2002-like epidemic of the 2003–2004 influenza season. However, despite sharing the same hemagglutinin, these phylogenetically distinct lineages of viruses continue to co-circulate in the same population. These data, derived from the first large-scale analysis of H3N2 viruses, convincingly demonstrate that multiple lineages can co-circulate, persist, and reassort in epidemiologically significant ways, and underscore the importance of genomic analyses for future influenza surveillance. Evolution of the flu virus is analyzed via genomic phylogeny; humans are found to provide a reservoir of antigenic variability implicit in flu adaptation and virulence.
登录
查看更多内容
影响因子：
64.8
作者：
BEAN, WJ;COX, NJ;KENDAL, AP
通讯作者：
KENDAL, AP
DOI：
10.1073/pnas.75.7.3341
发表时间：
1978-01-01
影响因子：
11.1
作者：
DESSELBERGER, U;NAKAJIMA, K;PALESE, P
通讯作者：
PALESE, P
DOI：
10.1073/pnas.94.15.7712
发表时间：
1997-07-22
影响因子：
11.1
作者：
Fitch, WM;Bush, RM;Cox, NJ
通讯作者：
Cox, NJ
DOI：
10.1073/pnas.88.10.4270
发表时间：
1991-05-01
影响因子：
11.1
作者：
FITCH, WM;LEITER, JME;PALESE, P
通讯作者：
PALESE, P
影响因子：
5.4
作者：
BEAN, WJ;SCHELL, M;WEBSTER, RG
通讯作者：
WEBSTER, RG