Recording of Electrically Evoked Neural Activity and Bladder Pressure Responses in Awake Rats Chronically Implanted With a Pelvic Nerve Array.
Recording of Electrically Evoked Neural Activity and Bladder Pressure Responses in Awake Rats Chronically Implanted With a Pelvic Nerve Array.
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DOI：
10.3389/fnins.2020.619275
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发表时间：
2020
影响因子：
4.3
通讯作者：
Fallon JB
中科院分区：
文献类型：
--
作者：
Payne SC;Wiedmann NM;Eiber CD;Wong AW;Senn P;Osborne PB;Keast JR;Fallon JB
生物电子医疗器械已被广泛应用于泌尿系统功能障碍的治疗。批准的靶点包括骶S3脊神经根和胫后神经，但另一个靶点是骨盆内脏神经组，因为这些神经包含协调膀胱储存和排尿功能的骶内脏感觉和自主运动通路。在这里，我们开发了一种适合于长期使用的设备在清醒的大鼠模型，研究电神经调节的骨盆神经（同源的人类骨盆内脏神经）。在雄性Sprague-Dawley大鼠中，将定制的平面四电极阵列植入骨盆神经远端，靠近主要骨盆神经节。电诱发复合动作电位（ECAP）在麻醉和长期植入，清醒的大鼠长达8周的手术后可靠地检测。ECAP波形显示三个峰，其中lavonium表明电刺激激活了有髓A纤维和无髓C纤维轴突的几个亚群。通过连续膀胱测压，阵列的慢性植入对清醒大鼠诱发的排尿没有影响，其中排尿参数与未处理大鼠中发表的参数相当。长期植入阵列的电刺激也诱导了两类膀胱压力反应，在清醒的大鼠中通过连续流膀胱测压检测：排尿收缩和非排尿收缩。通过免疫组织化学可视化神经元损伤或有害脊髓激活标记物，未检测到长期植入阵列产生的组织病理学证据。这些结果表明，大鼠骨盆神经电极阵列可用于临床前开发靶向骨盆神经的闭环神经调节装置，作为神经泌尿功能障碍的治疗方法。
Bioelectronic medical devices are well established and widely used in the treatment of urological dysfunction. Approved targets include the sacral S3 spinal root and posterior tibial nerve, but an alternate target is the group of pelvic splanchnic nerves, as these contain sacral visceral sensory and autonomic motor pathways that coordinate storage and voiding functions of the bladder. Here, we developed a device suitable for long-term use in an awake rat model to study electrical neuromodulation of the pelvic nerve (homolog of the human pelvic splanchnic nerves). In male Sprague-Dawley rats, custom planar four-electrode arrays were implanted over the distal end of the pelvic nerve, close to the major pelvic ganglion. Electrically evoked compound action potentials (ECAPs) were reliably detected under anesthesia and in chronically implanted, awake rats up to 8 weeks post-surgery. ECAP waveforms showed three peaks, with latencies that suggested electrical stimulation activated several subpopulations of myelinated A-fiber and unmyelinated C-fiber axons. Chronic implantation of the array did not impact on voiding evoked in awake rats by continuous cystometry, where void parameters were comparable to those published in naïve rats. Electrical stimulation with chronically implanted arrays also induced two classes of bladder pressure responses detected by continuous flow cystometry in awake rats: voiding contractions and non-voiding contractions. No evidence of tissue pathology produced by chronically implanted arrays was detected by immunohistochemical visualization of markers for neuronal injury or noxious spinal cord activation. These results demonstrate a rat pelvic nerve electrode array that can be used for preclinical development of closed loop neuromodulation devices targeting the pelvic nerve as a therapy for neuro-urological dysfunction.
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DOI：
10.1111/j.1748-1716.1990.tb08864.x
发表时间：
1990-03-01
期刊：
ACTA PHYSIOLOGICA SCANDINAVICA
影响因子：
--
作者：
ANDERSSON, PO;SJOGREN, C;UVNASMOBERG, K
通讯作者：
UVNASMOBERG, K
影响因子：
6.6
作者：
Chen, Mang L.;Chermansky, Christopher J.;Tai, Changfeng
通讯作者：
Tai, Changfeng
影响因子：
4
作者：
Crook JJ;Lovick TA
通讯作者：
Lovick TA
影响因子：
2.9
作者：
Forrest SL;Payne SC;Keast JR;Osborne PB
通讯作者：
Osborne PB
影响因子：
--
作者：
de Groat, William C;Yoshimura, Naoki
通讯作者：
Yoshimura, Naoki