Morphological evidence of an altered process of synaptic transcytosis in adult rats exposed to ethanol.
Morphological evidence of an altered process of synaptic transcytosis in adult rats exposed to ethanol.
复制标题
暴露于乙醇的成年大鼠突触转胞吞作用改变过程的形态学证据。
作者：
Y. Takeuchi;T. Miki;Jun;Ken;K. Warita;Y. Matsumoto;Shingo Suzuki;Motoki Tamai;K. Ameno;K. Bedi;Tomiko Yakura
目标
AIMS
The effects of ethanol exposure on synaptic structure were investigated in the nucleus of solitary tract (NST) in rats, using the horse-radish peroxidase (HRP) method.
METHODS
Eight-week-old experimental rats were allowed free access to a liquid diet containing ethanol for 3 weeks, while controls were given an isocaloric diet. Some of the control and experimental animals were given an injection of wheat germ agglutinin conjugated with HRP (WGA-HRP) into the vagus nerve toward the end of the treatment period. After the treatment, the neuropil region of the NST was examined under an electron microscope.
RESULTS
We observed that a few terminals were characterized by deep indentation of axodendritic membranes into the post-synaptic neurons. This appeared to be similar to that commonly seen in exocrine glands. Interestingly, the indented portion often contained various sizes of vacuoles and flattened cisternae. HRP-reaction product (RP) transported to terminals was recognized easily as an electron-dense lysosomal substance when lead citrate staining was omitted. Terminals containing HRP-RP also revealed quite a similar structure with indentation of axodendritic membranes as described earlier. The results are considered to confirm that terminals forming 'apocrine-like structures' observed in the ethanol-fed animals with no injection of WGA-HRP originate from afferent fibers of the vagus nerve.
CONCLUSION
The present study suggests the possibility that the alteration of the synaptic structure induced by ethanol exposure can lead to the neuronal transcytosis of materials including proteins which is different from the normal vesicular exocytosis involved in chemical synaptic transmission.
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影响因子：
4.2
作者：
Pardini, Dustin;White, Helene Raskin;Stouthamer-Loeber, Magda
通讯作者：
Stouthamer-Loeber, Magda
DOI：
10.1097/00004583-199601000-00018
发表时间：
1996-01-01
影响因子：
13.3
作者：
Rohde, P;Lewinsohn, PM;Seeley, JR
通讯作者：
Seeley, JR
DOI：
10.1111/j.1530-0277.1998.tb03651.x
发表时间：
1998-04-01
影响因子：
3.2
作者：
Mattson, SN;Riley, EP
通讯作者：
Riley, EP
主动脉压力感受器在乙醇引起的清醒大鼠心率压力反射控制受损中的作用。
DOI：
--
发表时间：
1992
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
el-Mas,MM;Abdel-Rahman,AA
通讯作者：
Abdel-Rahman,AA
长期乙醇和铁给药对大鼠小脑铁含量、神经元一氧化氮合酶和超氧化物歧化酶的影响。
DOI：
--
发表时间：
1999
期刊：
Alcoholism, clinical and experimental research.
影响因子：
--
作者：
Xia,J;Simonyi,A;Sun,GY
通讯作者：
Sun,GY