Characteristics of the binding of platelet-activating factor to platelets of different animal species.
Characteristics of the binding of platelet-activating factor to platelets of different animal species.
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血小板活化因子与不同动物种类血小板的结合特征。
DOI：
10.1016/0014-2999(84)90623-x
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发表时间：
1984
影响因子：
5
通讯作者：
M. Crespo
中科院分区：
文献类型：
--
作者：
P. Iñarrea;J. Gomez;M. Nieto;M. Crespo
血小板活化因子（PAF-acether）与人、兔和大鼠血小板的结合通过37°C用放射性标记的[3h] PAF-acether孵育洗净的血小板来研究。结合的Scatchard图分析表明，人和兔的血小板具有两种不同类型的结合位点。其中一种是饱和的，具有高亲和力（人的K d为1.58±0.36 nM，家兔的K d为0.9±0.5 rmnM），另一种具有几乎无限的结合能力。相比之下，大鼠血小板仅显示非特异性结合。在加入[3 H] paf -醚5分钟后，加入未标记的paf -醚，表明人血小板特异性结合的内化率为66±9%，兔血小板特异性结合的内化率为52±6.9%。兔血小板中[3h] paf -醚的特异性结合与不同剂量paf -醚对[3h]血清素的释放以及血小板对钙的吸收密切相关。相反，paf -醚不诱导大鼠血小板释放[3h]血清素或钙摄取。以下数据表明，paf -醚激活血小板的潜力取决于与特定膜受体的相互作用，而不是非受体介导的血小板膜改变：(1)对paf -醚敏感的动物血小板表现出结合的饱和性和特异性；(2)对paf -醚不敏感的动物血小板缺乏特异性结合；(3) paf -醚不能诱导血小板对钙的吸收，因为缺乏特定的结合位点。
The binding of platelet-activating factor (PAF-acether) to human, rabbit and rat platelets was investigated by incubating washed platelets at 37° C with radiolabeled [3 H] PAF-acether. Scatchard plot analysis of the binding showed that human and rabbit platelets possess two different types of binding sites. One of them was saturable and of high affinity (K d 1.58±0.36 nM in human and 0.9±0.5 rmnM in rabbits), and the other one had nearly infinite binding capacity. By contrast, rat platelets only showed non-specific binding. Addition of unlabeled PAF-acether 5 min after the addition of [3 H] PAF-acether showed that internalization of 66±9% of the specific binding in human and 52±6.9% in rabbit platelets had occurred. Specific binding of [3 H] PAF-acether in rabbit platelets correlated well with [3 H] serotonin release in response to different doses of PAF-acether and with the uptake of calcium by the platelets. By contrast, PAF-acether did not induce [3 H] serotonin release or calcium uptake by rat platelets. The following data suggest that the potential of PAF-acether to activate platelets depends on the interaction with a specific membrane receptor rather than on a non-receptor-mediated alteration of the platelet membrane:(1) platelets from animal species sensitive to PAF-acether show saturability and specificity of binding;(2) platelets from one animal species non-sensitive to PAF-acether lack specific binding;(3) PAF-acether does not induce calcium uptake by platelets from an animal species which lacks specific binding sites.
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改进的高效液相色谱方法用于从其他磷脂中分离血小板激活因子。
DOI：
10.1016/s0378-4347(00)80963-9
发表时间：
1983
期刊：
Journal of chromatography
影响因子：
--
作者：
Blank,ML;Snyder,F
通讯作者：
Snyder,F
影响因子：
--
作者：
FINDLAY, SR;LICHTENSTEIN, LM;PINCKARD, RN
通讯作者：
PINCKARD, RN
兔血小板活化对不同Ca2池的要求。
DOI：
10.1016/0304-4165(82)90159-3
发表时间：
1982
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Shaw,JO;Lyons,RM
通讯作者：
Lyons,RM
乙酰甘油醚磷酰胆碱与兔血小板相互作用的代谢行为。
影响因子：
3.9
作者：
Pieroni,G;Hanahan,DJ
通讯作者：
Hanahan,DJ
乙酰甘油醚磷酸胆碱诱导兔皮肤白细胞浸润。
DOI：
--
发表时间：
1982
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Humphrey,DM;Hanahan,DJ;Pinckard,RN
通讯作者：
Pinckard,RN