Genome-Wide Analysis of Terpene Synthase Gene Family in Mentha longifolia and Catalytic Activity Analysis of a Single Terpene Synthase.
Genome-Wide Analysis of Terpene Synthase Gene Family in Mentha longifolia and Catalytic Activity Analysis of a Single Terpene Synthase.
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DOI：
10.3390/genes12040518
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发表时间：
2021-04-02
期刊：
影响因子：
3.5
通讯作者：
Lange BM
中科院分区：
文献类型：
--
作者：
Chen Z;Vining KJ;Qi X;Yu X;Zheng Y;Liu Z;Fang H;Li L;Bai Y;Liang C;Li W;Lange BM
萜类化合物是一类种类繁多的天然产物，萜类合成酶（TPS）在萜类化合物的生物合成中起着关键作用。薄荷属植物中含有丰富的精油，其主要成分为萜类化合物，其生物合成途径已基本阐明。然而，目前对薄荷属植物中的TPS缺乏系统的鉴定和研究。在这项工作中，我们在全基因组范围内鉴定和分析了薄荷TPS基因家族，薄荷属功能基因组研究的模式植物。在M. longifolia基因组序列拼接，可分为6个亚科。其中TPS-b亚家族的基因数最多，可能与薄荷属植物中丰富的单萜类化合物有关。TPS-e亚科有18个成员，与已测序的唇形科植物相比，表现出明显的种特异性扩展。将63个TPS基因定位于M. longifolia基因组序列组装，这些基因的分布是不均匀的。串联重复和片段重复对M.长叶的分析了M中的保守基序RR（X）8 W、NSE/DTE、RXR和DDXXD。longifolia TPS，且不同亚科间存在显著分化。适应性进化分析表明，M.对长叶TPS进行种特异性扩增后进行纯化选择，并对正选择条件下的部分氨基酸残基进行鉴定。此外，我们还克隆和分析了一个单一的萜烯合酶，MlongTPS 29，它属于TPS-b亚家族的催化活性。MlongTPS 29基因编码柠檬烯合成酶，催化薄荷属植物精油的重要前体物质柠檬烯的生物合成。本研究为薄荷属植物萜类化合物的生物合成提供了有用的信息。
Terpenoids are a wide variety of natural products and terpene synthase (TPS) plays a key role in the biosynthesis of terpenoids. Mentha plants are rich in essential oils, whose main components are terpenoids, and their biosynthetic pathways have been basically elucidated. However, there is a lack of systematic identification and study of TPS in Mentha plants. In this work, we genome-widely identified and analyzed the TPS gene family in Mentha longifolia, a model plant for functional genomic research in the genus Mentha. A total of 63 TPS genes were identified in the M. longifolia genome sequence assembly, which could be divided into six subfamilies. The TPS-b subfamily had the largest number of genes, which might be related to the abundant monoterpenoids in Mentha plants. The TPS-e subfamily had 18 members and showed a significant species-specific expansion compared with other sequenced Lamiaceae plant species. The 63 TPS genes could be mapped to nine scaffolds of the M. longifolia genome sequence assembly and the distribution of these genes is uneven. Tandem duplicates and fragment duplicates contributed greatly to the increase in the number of TPS genes in M. longifolia. The conserved motifs (RR(X)8W, NSE/DTE, RXR, and DDXXD) were analyzed in M. longifolia TPSs, and significant differentiation was found between different subfamilies. Adaptive evolution analysis showed that M. longifolia TPSs were subjected to purifying selection after the species-specific expansion, and some amino acid residues under positive selection were identified. Furthermore, we also cloned and analyzed the catalytic activity of a single terpene synthase, MlongTPS29, which belongs to the TPS-b subfamily. MlongTPS29 could encode a limonene synthase and catalyze the biosynthesis of limonene, an important precursor of essential oils from the genus Mentha. This study provides useful information for the biosynthesis of terpenoids in the genus Mentha.
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影响因子：
3.1
作者：
Aubourg, S;Lecharny, A;Bohlmann, J
通讯作者：
Bohlmann, J
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
7.4
作者：
Jiang, Shu-Ye;Christoffels, Alan;Ramachandran, Srinivasan
通讯作者：
Ramachandran, Srinivasan
影响因子：
14.9
作者：
Letunic, Ivica;Bork, Peer
通讯作者：
Bork, Peer
DOI：
10.1007/10_2014_289
发表时间：
2015-01-01
期刊：
BIOTECHNOLOGY OF ISOPRENOIDS
影响因子：
--
作者：
Lange, Bernd Markus
通讯作者：
Lange, Bernd Markus