Environmental Sequencing Fills the Gap Between Parasitic Haplosporidians and Free-living Giant Amoebae.
Environmental Sequencing Fills the Gap Between Parasitic Haplosporidians and Free-living Giant Amoebae.
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DOI：
10.1111/jeu.12501
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发表时间：
2018-01-16
期刊：
影响因子：
--
通讯作者：
Bass D
中科院分区：
文献类型：
--
作者：
Ward GM;Neuhauser S;Groben R;Ciaghi S;Berney C;Romac S;Bass D
Ascetosporea纲（Rhizaria; Endomyxa）由许多无脊椎动物的寄生虫组成。在这一群体中，最近的群体特异性环境DNA （eDNA）研究有助于建立新的微胞虫目，新的副胞虫系统发育和特征，并阐明单倍孢子虫的多样性和分布。在这里，我们使用一般的和谱系特异性的PCR引物来研究单孢子虫和它们最接近的自由生活的亲戚——无卵阿米巴和网状阿米巴之间的系统发育“差距”。在这个间隙中是桡足类的寄生虫，我们发现它们高度多样化，广泛分布于浮游生物和底栖生物样本中。我们发现了一种强有力的寄生虫辐射，由Paradinium和另外三个分支（ENDO‐3a， ENDO‐3b和SPP）组成。另一个环境组，ENDO - 2，可能包括几个分支，介于这种辐射和自由生活的变形虫之间的分支。早期分化的单倍体也被扩增，通常与双壳类或深海样品有关。一般引物方法扩增了ENDO‐3和单倍孢子虫中重叠的一组新谱系，而群体特异性引物策略，旨在扩增已知最早的单倍孢子虫到Gromia，在部分系统发育范围内产生了更大的序列多样性。
Class Ascetosporea (Rhizaria; Endomyxa) comprises many parasites of invertebrates. Within this group, recent group‐specific environmental DNA (eDNA) studies have contributed to the establishment of the new order Mikrocytida, a new phylogeny and characterization of Paramyxida, and illuminated the diversity and distribution of haplosporidians. Here, we use general and lineage‐specific PCR primers to investigate the phylogenetic “gap” between haplosporidians and their closest known free‐living relatives, the testate amoeba Gromia and reticulate amoeba Filoreta. Within this gap are Paradinium spp. parasites of copepods, which we show to be highly diverse and widely distributed in planktonic and benthic samples. We reveal a robustly supported radiation of parasites, ENDO‐3, comprised of Paradinium and three further clades (ENDO‐3a, ENDO‐3b and SPP). A further environmental group, ENDO‐2, perhaps comprising several clades, branches between this radiation and the free‐living amoebae. Early diverging haplosporidians were also amplified, often associated with bivalves or deep‐sea samples. The general primer approach amplified an overlapping set of novel lineages within ENDO‐3 and Haplosporida, whereas the group‐specific primer strategy, targeted to amplify from the earliest known divergent haplosporidians to Gromia, generated greater sequence diversity across part of this phylogenetic range.
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影响因子：
56.9
作者：
Lima-Mendez, Gipsi;Faust, Karoline;Wincker, Patrick
通讯作者：
Wincker, Patrick
影响因子：
10.7
作者：
Katoh K;Standley DM
通讯作者：
Standley DM
DOI：
10.1073/pnas.1500163112
发表时间：
2015-08-25
影响因子：
11.1
作者：
Chambouvet, Aurelie;Gower, David J.;Richards, Thomas A.
通讯作者：
Richards, Thomas A.
影响因子：
11
作者：
Berney, Cedric;Romac, Sarah;Bass, David
通讯作者：
Bass, David
影响因子：
4.2
作者：
Chambouvet A;Berney C;Romac S;Audic S;Maguire F;De Vargas C;Richards TA
通讯作者：
Richards TA