Bioluminescent imaging of Borrelia burgdorferi in vivo demonstrates that the fibronectin-binding protein BBK32 is required for optimal infectivity.
Bioluminescent imaging of Borrelia burgdorferi in vivo demonstrates that the fibronectin-binding protein BBK32 is required for optimal infectivity.
复制标题
体内伯氏毛毛毛毛毛虫的生物发光成像表明，纤连蛋白结合蛋白BBK32是最佳感染性所必需的。
DOI：
10.1111/j.1365-2958.2011.07801.x
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发表时间：
2011-10
影响因子：
3.6
通讯作者：
Skare JT
中科院分区：
文献类型：
--
作者：
Hyde JA;Weening EH;Chang M;Trzeciakowski JP;Höök M;Cirillo JD;Skare JT
莱姆病的病原体伯氏疏螺旋体通过感染的硬蜱属传播。滴答声。感染，如果不治疗，导致传播到多个组织和显着的发病率。生物发光技术的最新发展允许在感染期间对病原生物体进行体内成像和定量。本文中，表达β-淀粉酶的B.通过生物发光成像对伯氏螺旋体和缺乏核心蛋白聚糖粘附素DbpA和DbpB以及纤连蛋白粘附素BBK 32的菌株进行定量，以进一步评估它们在感染小鼠中的致病潜力。通过定量PCR（qPCR）和培养验证细菌载量的定量。B。缺乏DbpA和DbpB的伯氏螺旋体仅在感染后1 h时间点观察到，与其低感染性表型一致。相对于其亲本，bbk 32突变体在第7天表现出其感染负荷的显著降低。这种效应在较低接种量下最明显，并且成像与qPCR数据良好相关。这些数据表明BBK 32介导的结合在B中起重要作用。伯氏殖民。因此，生物发光疏螺旋体的体内成像提供了在非侵入性生理相关环境中检测、定量和暂时表征疏螺旋体传播的灵敏手段，更重要的是，证明了bbk 32突变体的可定量感染性缺陷。
The etiologic agent of Lyme disease, Borrelia burgdorferi, is transmitted via infected Ixodes spp. ticks. Infection, if untreated, results in dissemination to multiple tissues and significant morbidity. Recent developments in bioluminescence technology allow in vivo imaging and quantification of pathogenic organisms during infection. Herein, luciferase-expressing B. burgdorferi and strains lacking the decorin adhesins DbpA and DbpB, as well as the fibronectin adhesin BBK32, were quantified by bioluminescent imaging to further evaluate their pathogenic potential in infected mice. Quantification of bacterial load was verified by quantitative PCR (qPCR) and cultivation. B. burgdorferi lacking DbpA and DbpB were only seen at the 1 h time point post-infection, consistent with its low infectivity phenotype. The bbk32 mutant exhibited a significant decrease in its infectious load at day 7 relative to its parent. This effect was most pronounced at lower inocula and imaging correlated well with qPCR data. These data suggest that BBK32-mediated binding plays an important role in B. burgdorferi colonization. As such, in vivo imaging of bioluminescent Borrelia provides a sensitive means to detect, quantify, and temporally characterize borrelial dissemination in a non-invasive, physiologically relevant environment and, more importantly, demonstrated a quantifiable infectivity defect for the bbk32 mutant.
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影响因子：
3.1
作者：
Labandeira-Rey, M;Skare, JT
通讯作者：
Skare, JT
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作者：
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