Effects of total soy saponins on free radicals in the quadriceps femoris, serum testosterone, LDH, and BUN of exhausted rats.
Effects of total soy saponins on free radicals in the quadriceps femoris, serum testosterone, LDH, and BUN of exhausted rats.
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DOI：
10.1016/j.jshs.2016.01.016
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发表时间：
2017-09
影响因子：
11.7
通讯作者：
Xiong Z
中科院分区：
文献类型：
--
作者：
Liu Z;Nie R;Liu Y;Li Z;Yang C;Xiong Z
研究大豆总皂苷(TS)对力竭运动大鼠股四头肌自由基代谢、血清睾酮、乳酸脱氢酶(LDH)和血尿素氮(BUN)的影响。采用一次性力竭跑步机锻炼。将SD大鼠随机分为4组：正常对照组(NTSNE)、TS不运动组(TSNE)、TS不运动组(NTSE)和TS运动组(TSE)。丹参总皂苷和丹参总皂苷按20 mg/kg体重灌胃，1次/d，连续2周。NTSE组给予安慰剂，NTSNE组不接受任何治疗。NTSE组和TSE组在跑台上以30min/m in的速度进行运动，直至力竭。记录大鼠疲劳时的运动时间和运动距离，立即断头解剖。制备10%的股四头肌组织匀浆。测定超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽还原酶(GR)、还原型谷胱甘肽(GSH)、总抗氧化能力(T-AOC)、乳酸脱氢酶(LDH)、尿素氮(BUN)和血清睾酮水平。TS显著增加运动至力竭的时间20.62%(p <； 0.05)。总抗氧化能力明显高于其他3组(p <； 0.0 1，p <； 0.0 1，p <； 0.0 5，p &l； 0.0 5)。丹参总皂苷组乳酸脱氢酶活力明显高于丹参总皂苷组(p <； 0.05)。与其他3组比较，NTSE组BUN水平显著升高(p <； 0.0 1，p <； 0.0 1，p <； 0.0 5)，且TSE组显著高于NTSNE组和TSNE组(p 和lt； 0.0 1)。治疗组血清睾酮水平显著高于其他3组(均p <； 0.01)。SOD、CAT、GSH-PX、GR和GSH在组间差异无统计学意义。TS能不同程度地提高运动大鼠血清睾酮水平和股四头肌抗氧化活性，降低丙二醛和血清LDH、BUN水平，延长运动时间，延缓疲劳的发生。
The aim of this study was to investigate the impact of total soy saponins (TS) on the free radical metabolism from the quadriceps femoris muscle, serum testosterone, lactate dehydrogenase (LDH), and blood urea nitrogen (BUN) in rats exercised to exhaustion. A one-time exhausted treadmill exercise session was used. Sprague-Dawley rats were divided into 4 groups: a control group—animals receiving no TS and no exercise (NTSNE), animals receiving TS but no exercise group (TSNE), animals receiving no TS but exercised to exhaustion group (NTSE), and animals receiving TS and exercised to exhaustion group (TSE). The TSNE and TSE groups were fed TS at a dosage of 20 mg/kg body weight once per day for 2 weeks. The NTSE group was given a placebo, and the NTSNE group was not given any treatment. The NTSE and TSE groups were exercised at speed of 30 m/min on treadmill until exhausted. The exercise time and exercise distance were recorded when the rats became exhausted and the rats were then decapitated and anatomized immediately. A 10% homogenate of the quadriceps femoris tissue was prepared. The levels of superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), glutathione reductase (GR), reduced glutathione (GSH), total antioxidant capacity (T-AOC), LDH, BUN, and serum testosterone were tested. TS significantly increased the exercise time to exhaustion by 20.62% (p < 0.05). The MDA levels were decreased significantly in the TSNE group than in NTSNE group (p < 0.05); the T-AOC levels increased significantly in the TSNE group than in the other 3 groups (p < 0.01, p < 0.05, p < 0.05). The LDH activity significantly increased in the NTSE group than in TSNE group (p < 0.05). The BUN levels significantly increased in the NTSE group than in the other 3 groups (p < 0.01, p < 0.01, p < 0.05), and significantly increased in the TSE group than in NTSNE and TSNE groups (both p < 0.01). The serum testosterone levels increased significantly in the TSNE group than in the other 3 groups (all p < 0.01). SOD, CAT, GSH-Px, GR, and GSH were not statistically different among the groups. TS can significantly improve the exercised rats' serum testosterone level and antioxidant activity in their quadriceps femoris to varying degrees, decrease MDA and serum LDH and BUN levels, increase the exercise time, and delay the occurrence of the fatigue.
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影响因子：
2.7
作者：
Hayashi, K;Hayashi, H;Ikeshiro, Y
通讯作者：
Ikeshiro, Y
影响因子：
120.7
作者：
SIMMONS, K
通讯作者：
SIMMONS, K
影响因子：
11.7
作者：
Liu Z;Liu Y;Xiong Z;Feng Y;Tang W
通讯作者：
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影响因子：
3
作者：
Huang, Chi-Chang;Lin, Tien-Jen;Lin, Wan-Teng
通讯作者：
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影响因子：
3.8
作者：
NAKASHIMA, H;OKUBO, K;YAMAMOTO, N
通讯作者：
YAMAMOTO, N