Progress in a replicated selection for elevated blood ethanol concentrations in HDID mice.
Progress in a replicated selection for elevated blood ethanol concentrations in HDID mice.
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DOI：
10.1111/gbb.12105
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发表时间：
2014-02
期刊：
影响因子：
--
通讯作者：
Phillips TJ
中科院分区：
文献类型：
--
作者：
Crabbe JC;Metten P;Belknap JK;Spence SE;Cameron AJ;Schlumbohm JP;Huang LC;Barkley-Levenson AM;Ford MM;Phillips TJ
黑暗饮酒（DID）是小鼠中一种有限的乙醇饮用表型。黑暗中高饮酒 (HDID-1) 小鼠经过 27 个选定世代 (S27) 的饲养，在接触 20% 乙醇 4 小时后血液乙醇浓度 (BEC) 升高。第二个复制品系 (HDID-2) 后来从相同的创始者群体开始，目前处于 S20。 S11 之后发布了 HDID-1 选择反应的初步报告。本文报告了这两个品系与 HS 对照的遗传和行为特征。两个重复的遗传力都很低 (h2 = 0.09)，但自 S0 以来，这些品系的 BEC 增加了 4-5 倍； 80% 的 HDID-1 和 60% 的 HDID-2 小鼠的 BEC 达到大于 1.0 mg/ml。 DID 测试几个小时后，HDID 小鼠表现出轻微的戒断迹象。尽管在选择过程中没有考虑，但 DID 测试期间的乙醇摄入量 (g/kg) 在 HDID-1 中增加了约 80%，在 HDID-2 中增加了约 60%。在确定 HDID 小鼠 BEC 和摄入量之间的相似性时，常见的遗传影响比环境影响更重要。对摄入量分配的分析表明，60%的摄入量集中在 4 小时训练的最后 2 小时。然而，这一点在选择过程中并没有改变。 DID 测试期间每小时的 BEC 在饮酒 3 或 4 小时后达到峰值水平。 HDID 小鼠与 HS 小鼠在消除急性剂量酒精的速度方面没有差异。
Drinking in the Dark (DID) is a limited access ethanol drinking phenotype in mice. High Drinking in the Dark (HDID-1) mice have been bred for 27 selected generations (S27) for elevated blood ethanol concentrations (BECs) after a 4 hr period of access to 20% ethanol. A second replicate line (HDID-2) was started later from the same founder population and is currently in S20. An initial report of response to selection in HDID-1 was published after S11. This paper reports genetic and behavioral characteristics of both lines in comparison with the HS controls. Heritability is low in both replicates (h2 = 0.09) but the lines have shown 4-5 fold increases in BEC since S0; 80% of HDID-1 and 60% of HDID-2 mice reach BECs greater than 1.0 mg/ml. Several hours after a DID test, HDID mice show mild signs of withdrawal. Although not considered during selection, intake of ethanol (g/kg) during the DID test increased by approximately 80% in HDID-1 and 60% in HDID-2. Common genetic influences were more important than environmental influences in determining the similarity between BEC and intake for HDID mice. Analysis of the partitioning of intake showed that 60% of intake is concentrated in the last 2 hr of the 4 hr session. However, this has not changed during selection. Hourly BECs during the DID test reach peak levels after 3 or 4 hr of drinking. HDID mice do not differ from HS mice in their rate of elimination of an acute dose of alcohol.
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DOI：
10.1016/j.pbb.2011.07.017
发表时间：
2011-11
期刊：
Pharmacology, biochemistry, and behavior
影响因子：
--
作者：
Bell RL;Rodd ZA;Smith RJ;Toalston JE;Franklin KM;McBride WJ
通讯作者：
McBride WJ
DOI：
10.1111/j.1530-0277.2012.01749.x
发表时间：
2012-08-01
影响因子：
3.2
作者：
Barkley-Levenson, Amanda M.;Crabbe, John C.
通讯作者：
Crabbe, John C.
DOI：
10.1111/j.1530-0277.2008.00718.x
发表时间：
2008-08-01
影响因子：
3.2
作者：
Ford, Matthew M.;Yoneyama, Naomi;Finn, Deborah A.
通讯作者：
Finn, Deborah A.
DOI：
10.1111/j.1530-0277.2001.tb02168.x
发表时间：
2001-11-01
影响因子：
3.2
作者：
Lê, AD;Israel, Y;Harding, S
通讯作者：
Harding, S
影响因子：
3.4
作者：
Barkley-Levenson AM;Cunningham CL;Smitasin PJ;Crabbe JC
通讯作者：
Crabbe JC