KIF2A silencing inhibits the proliferation and migration of breast cancer cells and correlates with unfavorable prognosis in breast cancer.
KIF2A silencing inhibits the proliferation and migration of breast cancer cells and correlates with unfavorable prognosis in breast cancer.
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DOI：
10.1186/1471-2407-14-461
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发表时间：
2014-06-21
期刊：
影响因子：
3.8
通讯作者：
Gong Y
中科院分区：
文献类型：
--
作者：
Wang J;Ma S;Ma R;Qu X;Liu W;Lv C;Zhao S;Gong Y
驱动蛋白家族成员2a（KIF 2A）是真核细胞中发现的一种马达蛋白，与多种人类癌症的发生和进展相关。研究了KIF 2A在乳腺癌肿瘤发生和进展中的作用。采用免疫组化染色、真实的RT-PCR和western blot检测乳腺癌组织及癌旁正常组织中KIF 2A的表达。使用Kaplan-Meier生存和多变量分析估计与KIF 2A表达相关的患者生存。采用乳腺癌细胞株MDA-MB-231，研究KIF 2A-siRNA转染后细胞的增殖、迁移和侵袭。同一患者癌组织中KIF 2A的表达高于癌旁正常组织（P < 0.05）。KIF 2A在有淋巴结转移和HER 2阳性的癌组织中的表达高于无淋巴结转移的癌组织（P < 0.05）。乳腺癌组织中KIF 2A表达水平与患者生存期呈负相关（P < 0.05）。多因素分析显示KIF 2A是乳腺癌预后的独立预测因子（OR：16.55，95% CI：2.216-123.631，P = 0.006）。KIF 2A基因沉默可抑制癌细胞的增殖、迁移和侵袭能力（P < 0.05）。KIF 2A可能在乳腺癌进展中起重要作用，并可能成为乳腺癌的一种新的预测和预后标志物。
Kinesin family member 2a (KIF2A), a type of motor protein found in eukaryotic cells, is associated with development and progression of various human cancers. The role of KIF2A during breast cancer tumorigenesis and progression was studied. Immunohistochemical staining, real time RT-PCR and western blot were used to examine the expression of KIF2A in cancer tissues and adjacent normal tissues from breast cancer patients. Patients’ survival in relation to KIF2A expression was estimated using the Kaplan–Meier survival and multivariate analysis. Breast cancer cell line, MDA-MB-231 was used to study the proliferation, migration and invasion of cells following KIF2A-siRNA transfection. The expression of KIF2A in cancer tissues was higher than that in normal adjacent tissues from the same patient (P < 0.05). KIF2A expression in cancer tissue with lymph node metastasis and HER2 positive cancer were higher than that in cancer tissue without (P < 0.05). A negative correlation was found between KIF2A expression levels in breast cancer and the survival time of breast cancer patients (P < 0.05). In addition, multivariate analysis indicated that KIF2A was an independent prognostic for outcome in breast cancer (OR: 16.55, 95% CI: 2.216-123.631, P = 0.006). The proliferation, migration and invasion of cancer cells in vitro were suppressed by KIF2A gene silencing (P < 0.05). KIF2A may play an important role in breast cancer progression and is potentially a novel predictive and prognostic marker for breast cancer.
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DOI：
10.1111/j.1524-4741.2011.01067.x
发表时间：
2011-05
期刊：
The breast journal
影响因子：
--
作者：
Ginsburg OM;Love RR
通讯作者：
Love RR
影响因子：
3.3
作者：
Kline-Smith, SL;Walczak, CE
通讯作者：
Walczak, CE
影响因子：
11.2
作者：
Tomonaga, T;Matsushita, K;Nomura, F
通讯作者：
Nomura, F
影响因子：
45.3
作者：
Harvey, JM;Clark, GM;Allred, DC
通讯作者：
Allred, DC
影响因子：
50.5
作者：
Malvezzi, M.;Bertuccio, P.;Negri, E.
通讯作者：
Negri, E.