Pathway analysis of the transcriptome and metabolome of salt sensitive and tolerant poplar species reveals evolutionary adaption of stress tolerance mechanisms.
Pathway analysis of the transcriptome and metabolome of salt sensitive and tolerant poplar species reveals evolutionary adaption of stress tolerance mechanisms.
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DOI：
10.1186/1471-2229-10-150
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发表时间：
2010-07-17
影响因子：
5.3
通讯作者：
Polle A
中科院分区：
文献类型：
--
作者：
Janz D;Behnke K;Schnitzler JP;Kanawati B;Schmitt-Kopplin P;Polle A
胡杨（Populus euphratica）是耐盐杨树，杨杨（Populus x canescens）是盐敏感杨树。由于胡杨对盐的转录响应性较低，我们假设与盐敏感杨树相比，胡杨表现出先天的应激保护基因激活。为了验证这一假设，采用全基因组微阵列分析和FT-ICR-MS代谢物谱分析方法，比较了胡杨（P. euphratica）和白杨（P. x canescens）成熟无胁迫叶片的转录组和代谢组。直接跨种比较两种杨树的转录组需要对数据集进行过滤。属于氧化石墨烯纤细类别的基因“线粒体”、“细胞壁”、“运输”、“能量代谢”和“次级代谢”显著富集，而“细胞核”、“RNA或DNA结合”、“激酶活性”和“转录因子活性”类别的基因在胡杨中与P. x canescens相比显着减少。在胡杨中没有发现应激相关基因普遍激活的证据。代谢组和转录组数据的通路分析表明，与P. x . canescens相比，胡杨的初级糖积累更强，糖醇产生途径的激活，次级代谢物的消耗更快。生理测量结果显示，胡杨的呼吸作用、单宁和可溶性酚含量以及葡萄糖和果糖的富集程度均高于黑木犀，这证实了途径分析的结果。胡杨不依赖于一般的过度表达应激途径来耐受盐胁迫。相反，它表现出渗透调节（糖和糖醇）、离子区隔化（钠、钾和其他代谢物转运体）和活性氧（酚类化合物）解毒的永久激活控制机制。胡杨对盐碱化环境的进化适应显然与细胞代谢的能量需求增加和转录调控的缺失有关。
Populus euphratica is a salt tolerant and Populus × canescens a salt sensitive poplar species. Because of low transcriptional responsiveness of P. euphratica to salinity we hypothesized that this species exhibits an innate activation of stress protective genes compared with salt sensitive poplars. To test this hypothesis, the transcriptome and metabolome of mature unstressed leaves of P. euphratica and P. × canescens were compared by whole genome microarray analyses and FT-ICR-MS metabolite profiling. Direct cross-species comparison of the transcriptomes of the two poplar species from phylogenetically different sections required filtering of the data set. Genes assigned to the GO slim categories 'mitochondria', 'cell wall', 'transport', 'energy metabolism' and 'secondary metabolism' were significantly enriched, whereas genes in the categories 'nucleus', 'RNA or DNA binding', 'kinase activity' and 'transcription factor activity' were significantly depleted in P. euphratica compared with P. × canescens. Evidence for a general activation of stress relevant genes in P. euphratica was not detected. Pathway analyses of metabolome and transcriptome data indicated stronger accumulation of primary sugars, activation of pathways for sugar alcohol production, and faster consumption of secondary metabolites in P. euphratica compared to P. × canescens. Physiological measurements showing higher respiration, higher tannin and soluble phenolic contents as well as enrichment of glucose and fructose in P. euphratica compared to P. × canescens corroborated the results of pathway analyses. P. euphratica does not rely on general over-expression of stress pathways to tolerate salt stress. Instead, it exhibits permanent activation of control mechanisms for osmotic adjustment (sugar and sugar alcohols), ion compartmentalization (sodium, potassium and other metabolite transporters) and detoxification of reactive oxygen species (phenolic compounds). The evolutionary adaptation of P. euphratica to saline environments is apparently linked with higher energy requirement of cellular metabolism and a loss of transcriptional regulation.
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影响因子：
14.9
作者：
Benson DA;Karsch-Mizrachi I;Lipman DJ;Ostell J;Sayers EW
通讯作者：
Sayers EW
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
27.5
作者：
Amtmann, Anna
通讯作者：
Amtmann, Anna
影响因子：
5.4
作者：
Cervera, MT;Storme, V;Boerjan, W
通讯作者：
Boerjan, W
影响因子：
11.6
作者：
Aharon, R;Shahak, Y;Galili, G
通讯作者：
Galili, G