Wolbachia strain wAlbB maintains high density and dengue inhibition following introduction into a field population of Aedes aegypti.
Wolbachia strain wAlbB maintains high density and dengue inhibition following introduction into a field population of Aedes aegypti.
复制标题
DOI：
10.1098/rstb.2019.0809
复制
发表时间：
2021-02-15
期刊：
影响因子：
--
通讯作者：
Sinkins SP
中科院分区：
文献类型：
--
作者：
Ahmad NA;Mancini MV;Ant TH;Martinez J;Kamarul GMR;Nazni WA;Hoffmann AA;Sinkins SP
携带 wAlbB 沃尔巴克氏体菌株的埃及伊蚊传播登革热病毒的能力降低。 wAlbB 已被引入野生AE。马来西亚吉隆坡多个野外地点的埃及伊蚊种群持续高频率存在了两年多，并显着降低了登革热发病率。尽管这些令人鼓舞的结果表明 wAlbB 释放可以成为一种有效的登革热控制策略，但长期成功取决于 wAlbB 维持高种群频率和病毒传播抑制，而两者都可能受到现场沃尔巴克氏体与宿主共同进化的影响。在这里，wAlbB 携带 Ae。与 wAlbB 实验室菌落相比，停止释放 20 个月后从现场收集的埃及伊蚊显示沃尔巴克氏体密度或组织分布变化没有减少。 wAlbB 菌株继续诱导完全单向细胞质不相容性，在实验室条件下表现出完美的母体传播，并保留其抑制登革热的能力。此外，现场收集的 wAlbB 系接受了马来西亚登革热患者血液的挑战，结果显示显着阻止了病毒向唾液腺的传播。这些结果表明 wAlbB 继续抑制当前在伊蚊野外种群中流行的登革热菌株。埃及伊蚊，并为持续扩大沃尔巴克氏菌 wAlbB 释放以控制登革热提供额外支持。本文是主题“蚊媒疾病的新型控制策略”的一部分。
Aedes aegypti mosquitoes carrying the wAlbB Wolbachia strain show a reduced capacity to transmit dengue virus. wAlbB has been introduced into wild Ae. aegypti populations in several field sites in Kuala Lumpur, Malaysia, where it has persisted at high frequency for more than 2 years and significantly reduced dengue incidence. Although these encouraging results indicate that wAlbB releases can be an effective dengue control strategy, the long-term success depends on wAlbB maintaining high population frequencies and virus transmission inhibition, and both could be compromised by Wolbachia–host coevolution in the field. Here, wAlbB-carrying Ae. aegypti collected from the field 20 months after the cessation of releases showed no reduction in Wolbachia density or tissue distribution changes compared to a wAlbB laboratory colony. The wAlbB strain continued to induce complete unidirectional cytoplasmic incompatibility, showed perfect maternal transmission under laboratory conditions, and retained its capacity to inhibit dengue. Additionally, a field-collected wAlbB line was challenged with Malaysian dengue patient blood, and showed significant blocking of virus dissemination to the salivary glands. These results indicate that wAlbB continues to inhibit currently circulating strains of dengue in field populations of Ae. aegypti, and provides additional support for the continued scale-up of Wolbachia wAlbB releases for dengue control. This article is part of the theme issue ‘Novel control strategies for mosquito-borne diseases’.
登录
查看更多内容
影响因子：
3.3
作者：
Cooper, Brandon S.;Vanderpool, Dan;Turelli, Michael
通讯作者：
Turelli, Michael
影响因子：
3.3
作者：
Lindsey ARI;Rice DW;Bordenstein SR;Brooks AW;Bordenstein SR;Newton ILG
通讯作者：
Newton ILG
WOLBACHIA菌株WAU在埃及埃及伊德斯（Aedes Aegypti）中提供了高效的病毒传递阻滞。
影响因子：
6.7
作者：
Ant TH;Herd CS;Geoghegan V;Hoffmann AA;Sinkins SP
通讯作者：
Sinkins SP
影响因子：
3.8
作者：
Frentiu FD;Zakir T;Walker T;Popovici J;Pyke AT;van den Hurk A;McGraw EA;O'Neill SL
通讯作者：
O'Neill SL
影响因子：
2.6
作者：
Ant, T. H.;Herd, C.;Sinkins, S. P.
通讯作者：
Sinkins, S. P.