The Wolbachia strain wAu provides highly efficient virus transmission blocking in Aedes aegypti.
The Wolbachia strain wAu provides highly efficient virus transmission blocking in Aedes aegypti.
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WOLBACHIA菌株WAU在埃及埃及伊德斯（Aedes Aegypti）中提供了高效的病毒传递阻滞。
DOI：
10.1371/journal.ppat.1006815
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发表时间：
2018-01
期刊：
影响因子：
6.7
通讯作者：
Sinkins SP
中科院分区：
文献类型：
--
作者：
Ant TH;Herd CS;Geoghegan V;Hoffmann AA;Sinkins SP
引入遗传细菌沃尔巴克氏体的交叉感染可以抑制伊蚊的病毒传播，并且在伊蚊中。埃及伊蚊目前正在一些国家用于登革热控制。迄今为止，自然界中大量存在的沃尔巴克氏菌只有三种菌株被引入并在该物种中进行了鉴定。这里是小说Ae。使用wAlbA和wAu菌株产生埃及伊蚊交叉感染。在它的母语Ae。在白纹伊蚊中，wAlbA维持在较低的密度，但在转移到伊蚊后。埃及伊蚊的相对菌株密度相反，说明沃尔巴克氏体与宿主共同适应在确定密度方面具有菌株特异性。wAu菌株在伊蚊中也有较高的密度。并对登革热和寨卡病毒提供了高效的传播阻断。与wMel相比，wAu和wAlbA对饲养温度升高导致的密度降低/不完全母体传播的易感程度较低。虽然wAu不诱导细胞质不相容（CI），但它与一个诱导CI的菌株作为重复感染稳定地结合在一起，这将有助于其在野生种群中传播。沃尔巴克氏体wAu为虫媒病毒控制提供了一个非常有前途的新选择，特别是在炎热的热带气候中部署。蚊媒病毒性疾病对全球人类和动物健康构成日益严重的威胁。埃及伊蚊是登革热、寨卡病毒和基孔肯雅病毒等最重要的人类虫媒病毒感染的主要媒介，具有高度侵入性，在热带城市地区几乎无处不在。蚊虫控制仍然是预防和控制疫情的主要方法。一些国家目前正在试验一种新的控制策略。埃及伊蚊被人工感染了一种被称为沃尔巴克氏体的细菌共生体。虽然许多昆虫物种携带原生沃尔巴克氏体感染，但伊蚊。埃及伊蚊自然不受感染。沃尔巴克氏体存在于宿主细胞内，可由母体传给后代，并可阻断病毒传播；一旦被释放，它就会入侵并在宿主种群中持续存在。本文报道了两种新型沃尔巴克氏菌在伊蚊中的感染和鉴定。蚊。我们发现，其中一种菌株wAu对登革热和寨卡病毒的传播具有特别强的阻断作用，与目前最广泛用于野外释放的菌株well相比，它在较高温度下具有更大的稳定性。这些结果表明，wAu是虫媒病毒控制的有希望的选择，特别是在炎热气候下。
Introduced transinfections of the inherited bacteria Wolbachia can inhibit transmission of viruses by Aedes mosquitoes, and in Ae. aegypti are now being deployed for dengue control in a number of countries. Only three Wolbachia strains from the large number that exist in nature have to date been introduced and characterized in this species. Here novel Ae. aegypti transinfections were generated using the wAlbA and wAu strains. In its native Ae. albopictus, wAlbA is maintained at lower density than the co-infecting wAlbB, but following transfer to Ae. aegypti the relative strain density was reversed, illustrating the strain-specific nature of Wolbachia-host co-adaptation in determining density. The wAu strain also reached high densities in Ae. aegypti, and provided highly efficient transmission blocking of dengue and Zika viruses. Both wAu and wAlbA were less susceptible than wMel to density reduction/incomplete maternal transmission resulting from elevated larval rearing temperatures. Although wAu does not induce cytoplasmic incompatibility (CI), it was stably combined with a CI-inducing strain as a superinfection, and this would facilitate its spread into wild populations. Wolbachia wAu provides a very promising new option for arbovirus control, particularly for deployment in hot tropical climates. Mosquito-borne viral diseases represent an increasing threat to human and animal health globally. The mosquito species Aedes aegypti, a primary vector of the most significant human arboviral infections including the dengue, Zika and Chikungunya viruses, is highly invasive and is almost ubiquitous in tropical urban areas. Mosquito control remains the main approach for preventing and controlling outbreaks. A novel control strategy that is currently being trialed in several countries utilizes Ae. aegypti mosquitoes artificially infected with a bacterial symbiont known as Wolbachia pipientis. Although many insect species harbor native Wolbachia infections, Ae. aegypti is naturally uninfected. Wolbachia lives within host cells and is passed-on from mother to offspring, and can block virus transmission; once released it can invade and persist in host populations. Here we present the infection and assessment of two novel Wolbachia strains in Ae. aegypti. We show that one of the strains, wAu, provides particularly strong blocking of dengue and Zika virus transmission and offers greater stability at higher temperatures when compared to wMel—currently the most widely used strain for field releases. These results suggest that wAu is promising option for arbovirus control, especially in hot climates.
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影响因子：
6.7
作者：
Kriesner P;Hoffmann AA;Lee SF;Turelli M;Weeks AR
通讯作者：
Weeks AR
影响因子：
3.8
作者：
Frentiu FD;Zakir T;Walker T;Popovici J;Pyke AT;van den Hurk A;McGraw EA;O'Neill SL
通讯作者：
O'Neill SL
影响因子：
3.8
作者：
Hoffmann AA;Iturbe-Ormaetxe I;Callahan AG;Phillips BL;Billington K;Axford JK;Montgomery B;Turley AP;O'Neill SL
通讯作者：
O'Neill SL
影响因子：
4.1
作者：
Hoffmann AA;Ross PA;Rašić G
通讯作者：
Rašić G
影响因子：
2.6
作者：
Dutton, TJ;Sinkins, SP
通讯作者：
Sinkins, SP