Limited dengue virus replication in field-collected Aedes aegypti mosquitoes infected with Wolbachia.
Limited dengue virus replication in field-collected Aedes aegypti mosquitoes infected with Wolbachia.
复制标题
DOI：
10.1371/journal.pntd.0002688
复制
发表时间：
2014-02
影响因子：
3.8
通讯作者：
O'Neill SL
中科院分区：
文献类型：
--
作者：
Frentiu FD;Zakir T;Walker T;Popovici J;Pyke AT;van den Hurk A;McGraw EA;O'Neill SL
登革热是世界上传播最广泛的蚊媒疾病之一。病原体登革热病毒（DENV）主要由埃及伊蚊传播，这一物种已被证明难以用常规方法控制。埃及伊蚊与沃尔巴克氏体wMel菌株的交叉感染显示出有限的DENV复制，这一发现导致在澳大利亚凯恩斯试验田释放这些蚊子，作为该病毒的生物控制策略。与未感染的对照组相比，现场收集的三种DENV血清型攻击的wMel蚊子显示出有限的身体感染、病毒复制和头部传播率。登革热在野外蚊子中的感染、复制和传播率与在实验室中保持的原始感染的蚊子中观察到的相似。结果表明，wMel在野外蚊体组织中的分布与实验室蚊体相似，但在吸血后第7天，wMel在野外蚊体中的密度增加幅度较大。我们的研究结果表明，感染沃尔巴克氏体的蚊子在被释放并在野生种群中定居后，病毒阻断可能会持续存在，这表明沃尔巴克氏体生物防治可能是减少登革热在野外传播的一种成功策略。世界上几乎一半的人口面临感染登革热病毒的风险，特别是在热带和亚热带地区。这种病毒是由埃及伊蚊传播的，这是一种广泛存在的物种，事实证明很难用传统方法控制它。已经制定了一项新的生物防治策略，涉及释放感染沃尔巴克氏菌的蚊子。在实验室试验中，携带沃尔巴克氏体wMel菌株的蚊子显示登革热病毒的复制和传播显著减少。尽管前景光明，但沃尔巴克氏体生物防治的效用取决于野外感染沃尔巴克氏体的蚊子是否能保持病毒复制减少的表型。2011年初，在澳大利亚凯恩斯的野外释放了携带wMel的蚊子。我们提供的证据表明，一年后，与对照相比，现场收集的wMel蚊子在体内的登革热病毒复制减少，向头部的传播有限。吸血后，蚊子体内的沃尔巴克氏体数量增加，如果蚊子频繁进食，可能会进一步减少病毒的复制。我们的研究结果表明，沃尔巴克氏体介导的登革热干扰在野外种群中持续存在，并且在部署一年后没有显示出衰减的迹象。
Dengue is one of the most widespread mosquito-borne diseases in the world. The causative agent, dengue virus (DENV), is primarily transmitted by the mosquito Aedes aegypti, a species that has proved difficult to control using conventional methods. The discovery that A. aegypti transinfected with the wMel strain of Wolbachia showed limited DENV replication led to trial field releases of these mosquitoes in Cairns, Australia as a biocontrol strategy for the virus. Field collected wMel mosquitoes that were challenged with three DENV serotypes displayed limited rates of body infection, viral replication and dissemination to the head compared to uninfected controls. Rates of dengue infection, replication and dissemination in field wMel mosquitoes were similar to those observed in the original transinfected wMel line that had been maintained in the laboratory. We found that wMel was distributed in similar body tissues in field mosquitoes as in laboratory ones, but, at seven days following blood-feeding, wMel densities increased to a greater extent in field mosquitoes. Our results indicate that virus-blocking is likely to persist in Wolbachia-infected mosquitoes after their release and establishment in wild populations, suggesting that Wolbachia biocontrol may be a successful strategy for reducing dengue transmission in the field. Almost half of the world's population is at risk of contracting dengue virus, particularly in the tropics and sub-tropics. The virus is transmitted by the mosquito Aedes aegypti, a cosmopolitan species that has proved difficult to control using traditional methods. A new biocontrol strategy has been developed involving the release of mosquitoes infected with Wolbachia bacteria. Mosquitoes with the wMel strain of Wolbachia show dramatically reduced replication and transmission of dengue virus in laboratory trials. Although promising, the utility of Wolbachia biocontrol depends on field wMel-infected mosquitoes retaining the phenotype of reduced viral replication. Mosquitoes with wMel were released in the field in Cairns, Australia in early 2011. We provide evidence that, one year later, field collected wMel mosquitoes showed reduced dengue virus replication in the body and limited dissemination to the head compared to controls. Wolbachia numbers in mosquitoes increased following blood meals, which may further decrease viral replication if the insects feed frequently. Our results indicate that Wolbachia-mediated dengue interference is sustained in field populations and shows no sign of attenuation after one year of deployment.
登录
查看更多内容
影响因子：
6.7
作者：
Caragata EP;Rancès E;Hedges LM;Gofton AW;Johnson KN;O'Neill SL;McGraw EA
通讯作者：
McGraw EA
影响因子：
3.7
作者：
Frentiu FD;Robinson J;Young PR;McGraw EA;O'Neill SL
通讯作者：
O'Neill SL
影响因子：
2.6
作者：
Dutton, TJ;Sinkins, SP
通讯作者：
Sinkins, SP
DOI：
10.1073/pnas.052466499
发表时间：
2002-03-05
影响因子：
11.1
作者：
McGraw, EA;Merritt, DJ;O'Neill, SL
通讯作者：
O'Neill, SL
影响因子：
64.5
作者：
Moreira, Luciano A.;Iturbe-Ormaetxe, Inaki;O'Neill, Scott L.
通讯作者：
O'Neill, Scott L.